Optimized indirect 76Br-bromination of antibodies using N-succinimidyl para-[76Br]bromobenzoate for radioimmuno PET

被引：20

作者：

Höglund, J ^{[1
]}

Tolmachev, V

Orlova, A

Lundqvist, H

Sundin, A

机构：

[1] Univ Uppsala, Div Biomed Radiat Sci, Rudbeck Lab, S-75185 Uppsala, Sweden

[2] Univ Uppsala, Div Radiol, S-75185 Uppsala, Sweden

[3] Univ Uppsala, Dept Oncol Radiol & Clin Immunol, S-75185 Uppsala, Sweden

来源：

NUCLEAR MEDICINE AND BIOLOGY | 2000年 / 27卷 / 08期

关键词：

Br-76; N-succinimidyl para-[Br-76]bromobenzoate; PET; indirect labeling; monoclonal antibody;

D O I：

10.1016/S0969-8051(00)00153-0

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

Monoclonal antibody 38S1 was radiobrominated with the positron emitter Br-76 (T-1/2 - 16.2 h). Indirect labeling was performed using N-succinimidyl para (tri-methylstannyl) benzoate (SPMB) as the precursor molecule. SPMB was labeled using Chloramine-T yielding N-succinimidyl para-[Br-76]bromobenzoate, which was then conjugated to the antibody. Optimization of the labeling conditions and further conjugation gave a total yield (mean +/- max error) of 49 +/- 2%. The immunoreactivity of the antibodies was retained after labeling. Thus, antibodies intended for positron emission tomography can be labeled with Br-76, which gives high yields and preserved immunoreactivity when using the SPMB technique described. NUCL MED BIOL 27;8:837-843, 2000. (C) 2000 Elsevier Science Inc. All rights reserved.

引用

页码：837 / 843

页数：7

共 30 条

[1]

Browne E., 1986, Table of Radioactive Isotopes

[2]

GARG PK, 1989, APPL RADIAT ISOTOPES, V40, P485

[3] PRODUCTION OF CARRIER-FREE GA-66 AND LABELING OF ANTIMYOSIN ANTIBODY FOR POSITRON IMAGING OF ACUTE MYOCARDIAL-INFARCTION [J].

GOETHALS, P ;

COENE, M ;

SLEGERS, G ;

VOGELAERS, D ;

EVERAERT, J ;

LEMAHIEU, I ;

COLARDYN, F ;

HEYNDRICKX, GR .

EUROPEAN JOURNAL OF NUCLEAR MEDICINE, 1990, 16 (4-6) :237-240

[4]

Goldenberg D. M., 1992, International Journal of Biological Markers, V7, P183

[5] SPECIFICITIES AND BINDING-PROPERTIES OF EIGHT MONOCLONAL-ANTIBODIES AGAINST CARCINOEMBRYONIC ANTIGEN [J].

HEDIN, A ;

HAMMARSTROM, S ;

LARSSON, A .

MOLECULAR IMMUNOLOGY, 1982, 19 (12) :1641-1648

[6] POSITRON EMISSION TOMOGRAPHY OF MONOCLONAL-ANTIBODIES [J].

KAIREMO, KJA .

ACTA ONCOLOGICA, 1993, 32 (7-8) :825-830

[7]

Knight L.C., 1975, RADIOPHARMACEUTICALS, P149

[8]

KNIGHT LC, 1977, J NUCL MED, V18, P282

[9] SITES OF DIRECT AND INDIRECT HALOGENATION OF ALBUMIN [J].

KNIGHT, LC ;

WELCH, MJ .

BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 534 (02) :185-195

[10] A new convenient route to radioiodinated N-succinimidyl 3- and 4-iodobenzoate, two reagents for radioiodination of proteins [J].

Koziorowski, J ;

Henssen, C ;

Weinreich, R .

APPLIED RADIATION AND ISOTOPES, 1998, 49 (08) :955-959

← 1 2 3 →