Quantitative transcriptional profiling of ATDC5 mouse progenitor cells during chondrogenesis

被引:38
作者
Chen, L
Fink, T
Zhang, XY
Ebbesen, P
Zachar, V
机构
[1] Univ Aalborg, Dept Hlth Sci & Technol, Lab Stem Cell Res, DK-9220 Aalborg, Denmark
[2] Jiangsu Prov Hosp, Dept Hematol, Nanjing 210029, Peoples R China
关键词
chondrogenic differentiation; time-course expression; real-time RT-PCR; expression profile; cluster analysis;
D O I
10.1111/j.1432-0436.2005.00038.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
During the differentiation of a mouse chondroprogenitor cell line, ATDC5, an analysis of the transcription cartilage-related genes was carried out using real-time RT-PCR in a semiquantitative fashion. A total number of 104 genes both previously linked to chondrogenesis and hitherto not associated with the development of cartilage were analyzed. Parametric statistics, and unsupervised hierarchical and K-medians clustering approaches were used to analyze the gene expression during the sequential processes of proliferation, condensation, differentiation, maturation, and hypertrophic conversion of ATDC5 cells. The obtained data provided a robust determination of expression patterns that make possible an accurate assessment of the molecular events along the chondrogenic differentiation pathway. In addition, time-course expression profiles were described for eight highly regulated genes that have not been associated with chondrogenesis as yet. These included Cryab, Rcor2, Hig1, Bnip3, Mst4, Calml4, Gng2, and Islr.
引用
收藏
页码:350 / 363
页数:14
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