An inter-laboratory validation of methods of lipid peroxidation measurement in UVA-treated human plasma samples

被引：54

作者：

Breusing, Nicolle ^{[2
]}

Grune, Tilman ^{[2
]}

Andrisic, Luka ^{[3
]}

Atalay, Mustafa ^{[4
]}

Bartosz, Grzegorz ^{[5
]}

Biasi, Fiorella ^{[6
]}

Borovic, Suzana ^{[3
]}

Bravo, Laura ^{[7
]}

Casals, Isidre ^{[8
]}

Casillas, Rosario ^{[9
]}

Dinischiotu, Anca ^{[10
]}

Drzewinska, Joanna ^{[5
]}

Faber, Heidemarie ^{[11
]}

Fauzi, Norsyahida Mohd ^{[1
]}

Gajewska, Agnieszka ^{[5
]}

Gambini, Juan ^{[12
]}

Gradinaru, Daniela ^{[13
]}

Kokkola, Tarja ^{[4
]}

Lojek, Antonin ^{[14
]}

Luczaj, Wojciech ^{[15
]}

Margina, Denisa ^{[13
]}

Mascia, Cinzia ^{[6
]}

Mateos, Raquel ^{[7
]}

Meinitzer, Andreas ^{[16
]}

Teresa Mitjavila, Maria ^{[9
]}

Mrakovcic, Lidija ^{[3
]}

Munteanu, Maria Cristina ^{[10
]}

Podborska, Martina ^{[14
]}

Poli, Giuseppe ^{[6
]}

Sicinska, Paulina ^{[5
]}

Skrzydlewska, Elzbieta ^{[15
]}

Vina, Jose ^{[12
]}

Wiswedel, Ingrid ^{[11
]}

Zarkovic, Neven ^{[3
]}

Zelzer, Sieglinde ^{[16
]}

Spickett, Corinne M. ^{[1
]}

机构：

[1] Univ Strathclyde, Strathclyde Inst Pharm & Biomed Sci, Glasgow G4 0NR, Lanark, Scotland

[2] Univ Hohenheim, Inst Biol Chem & Nutr, D-7000 Stuttgart, Germany

[3] Rudjer Boskovic Inst, Zagreb, Croatia

[4] Univ Eastern Finland, Inst Biomed, Kuopio, Finland

[5] Univ Lodz, Dept Mol Biophys, PL-90131 Lodz, Poland

[6] Univ Turin, Dept Clin & Biol Sci, Turin, Italy

[7] Inst Frio ICTAN CSIC, Dept Metab & Nutr, Madrid, Spain

[8] Univ Barcelona, Serv Cient Tecn, Barcelona, Spain

[9] Univ Barcelona ISC III, Fac Biol, Dept Physiol, Barcelona, Spain

[10] Univ Bucharest, Dept Biochem & Mol Biol, Bucharest, Romania

[11] Otto VonGuericke Univ Magdegurg, Fac Med, Dept Pathobiochem, D-39016 Magdeburg, Germany

[12] Univ Valencia, Dept Physiol, Fac Med, E-46003 Valencia, Spain

[13] Carol Davila Univ Med & Pharm, Fac Pharm, Dept Biochem, Bucharest, Romania

[14] AS CR, Inst Biophys, Brno, Czech Republic

[15] Med Univ Bialystok, Dept Analyt Chem, Bialystok, Poland

[16] Med Univ Graz, Clin Inst Med & Chem Lab Diagnost, Graz, Austria

来源：

FREE RADICAL RESEARCH | 2010年 / 44卷 / 10期

关键词：

Oxidative stress; F2-isoprostanes; 4-hydroxynonenal; malondialdehyde; CHROMATOGRAPHY-MASS-SPECTROMETRY; PERFORMANCE LIQUID-CHROMATOGRAPHY; THIOBARBITURIC ACID ADDUCT; CORONARY-ARTERY-DISEASE; STRESS IN-VIVO; OXIDATIVE STRESS; MALONDIALDEHYDE; F-2-ISOPROSTANES; MARKERS; ATHEROSCLEROSIS;

D O I：

10.3109/10715762.2010.499907

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lipid peroxidation products like malondialdehyde, 4-hydroxynonenal and F-2-isoprostanes are widely used as markers of oxidative stress in vitro and in vivo. This study reports the results of a multi-laboratory validation study by COST Action B35 to assess inter-laboratory and intra-laboratory variation in the measurement of lipid peroxidation. Human plasma samples were exposed to UVA irradiation at different doses (0, 15 J, 20 J), encoded and shipped to 15 laboratories, where analyses of malondialdehyde, 4-hydroxynonenal and isoprostanes were conducted. The results demonstrate a low within-day-variation and a good correlation of results observed on two different days. However, high coefficients of variation were observed between the laboratories. Malondialdehyde determined by HPLC was found to be the most sensitive and reproducible lipid peroxidation product in plasma upon UVA treatment. It is concluded that measurement of malondialdehyde by HPLC has good analytical validity for inter-laboratory studies on lipid peroxidation in human EDTA-plasma samples, although it is acknowledged that this may not translate to biological validity.

引用

页码：1203 / 1215

页数：13

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