Toxicological effects in rats chronically fed low dietary levels of fumonisin B1

被引:36
作者
Gelderblom, WCA
Lebepe-Mazur, S
Snijman, PW
Abel, S
Swanevelder, S
Kriek, NPJ
Marasas, WFO
机构
[1] Programme Mycotoxins & Expt Carcinogenesis, ZA-7505 Tygerberg, South Africa
[2] S African MRC, ZA-7505 Tygerberg, South Africa
[3] Univ Pretoria, Fac Vet Sci, Dept Pathol, ZA-0110 Onderstepoort, South Africa
关键词
toxicity of fumonisin B(1) in rats;
D O I
10.1016/S0300-483X(00)00459-5
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The toxicity of low dietary levels of fumonisin B(1) (FB(1)), i.e. 1, 10 and 25 mg FB,;kg diet, were monitored in rats over a period of 24 months. No effects on the body weight gain and feed intake profiles were noticed, while the relative liver weight was significantly (P < 0.05) reduced in the FB(1)-treated rats. Mild toxic effects, including single cell necrosis (apoptosis). proliferation of bile duct epithelial cells (DEC), and early signs of fibrosis, bile duct hyperplasia and in one case, adenofibrosis, were noticed in the liver of the rats fed the highest (25 mg/FB(1)/kg diet) dietary level. A significant (P < 0.05) increase in the level of oxidative damage was also noticed in the liver of the rats of high dosage dietary group. The toxic effects were less severe in the 10 mg FB(1)/kg dietary group, whilst only a few ground glass foci were observed in the 1 mg FB(1)/kg dietary group. Hepatocyte nodules, staining positively for glutathione-S-transferase (placental form, PGST), were observed macroscopically in the 25 mg FB(1)/kg treated group and to a lesser extent in the 10 mg FB(1)/kg treated rats. The most prominent toxic lesions by FB(1) (10 and 25 mg FB(1)/kg dietary groups) in the kidneys were restricted to the tubular epithelium manifesting as granular cast, necrosis, apoptosis, calcification and the presence of regenerative foci in the proximal convoluted tubules. The existence of a cytotoxic/proliferative threshold with respect to cancer induction by FB, in rat liver became apparent, with a dietary level of < 10-mg FB(1)/ikg diet as a no effect threshold for the induction of hepatocyte nodules. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:39 / 51
页数:13
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