Differentially Expressed Protein Profile of Renal Tubule Cell Stimulated by Elevated Uric Acid Using SILAC Coupled to LC-MS

被引：26

作者：

Quan, Hong ^{[1
]}

Peng, Xue ^{[2
]}

Liu, Shuwen ^{[1
]}

Bo, Fu ^{[1
]}

Yang, Lv ^{[1
]}

Huang, Zhiyong ^{[1
]}

Li, Haixia

Chen, Xiangmei ^{[1
]}

Di, Wu ^{[1
]}

机构：

[1] Gen Hosp Chinese PLA, Inst Nephrol Chinese PLA, Key Kidney Dept, Clin Dept Internal Med, Beijing 100853, Peoples R China

[2] Chinese Acad Sci, Inst Biophys, Lab Prote, Beijing 100080, Peoples R China

来源：

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | 2011年 / 27卷 / 01期

基金：

中国国家自然科学基金;

关键词：

SILAC; Uric Acid; Renal tubule epithelial cell; PROLIFERATION; APOPTOSIS; S100A6; HYPERTENSION; CYCLE; CHROMATOGRAPHY; INJURY; HDAC1;

D O I：

10.1159/000325209

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Background/Aims: Hyperuricemia could lead to serious renal disease. It will advance our understanding of the mechanism of this disease to study the differentially expressed protein in renal tubular epithelial cells stimulated by elevated uric acid. Methods: We used SILAC coupled to LC-MS to study differentially expressed protein profile and to analysis the functional status of renal tubular epithelial cells stimulated by 600 mu M uric acid, and to investigate the potential biology function. The MS results were analyzed by online platform and further confirmed by western blotting. Results: 789 differentially expressed proteins were identified, of which 42 proteins and 49 proteins were related with cell proliferation and cell apoptosis, respectively. Pathway analysis showed that MAPK signaling pathway was a key pathway related to the function of these proteins. In addition, prohibitin-2 was identified to be related to renal cell transdifferentiation stimulated by elevated uric acid. Conclusion: This work provides an overview of protein expression changes in HK-2 cells treated with elevated uric acid and will contribute to further study of cell apoptosis and fibrosis in renal disease patients. Copyright (C) 2011 S. Karger AG, Basel

引用

页码：91 / 98

页数：8

共 41 条

[1]

Apweiler R, 2004, NUCLEIC ACIDS RES, V32, pD115, DOI [10.1093/nar/gkh131, 10.1093/nar/gkw1099]

[2] Annexin I is an endogenous ligand that mediates apoptotic cell engulfment [J].

Arur, S ;

Uche, UE ;

Rezaul, K ;

Fong, M ;

Scranton, V ;

Cowan, AE ;

Mohler, W ;

Han, DK .

DEVELOPMENTAL CELL, 2003, 4 (04) :587-598

[3] Expression of the RNA-stabilizing protein HuR in ischemia-reperfusion injury of rat kidney [J].

Ayupova, Dina A. ;

Singh, Mamata ;

Leonard, Ellen C. ;

Basile, David P. ;

Lee, Beth S. .

AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 2009, 297 (01) :F95-F105

[4] REQUIEM FOR GOUTY NEPHROPATHY [J].

BECK, LH ;

HARRINGTON, JT ;

KASSIRER, JP ;

PERRONE, R ;

MADIAS, NE ;

STROM, J ;

KURTIN, P ;

CANZANELLO, V .

KIDNEY INTERNATIONAL, 1986, 30 (02) :280-287

[5] Expression of keratin K2e in cutaneous and oral lesions - Association with keratinocyte activation, proliferation, and keratinization [J].

Bloor, BK ;

Tidman, N ;

Leigh, IM ;

Odell, E ;

Dogan, B ;

Wollina, U ;

Ghali, L ;

Waseem, A .

AMERICAN JOURNAL OF PATHOLOGY, 2003, 162 (03) :963-975

[6] A two step fractionation approach for plasma proteomics using immunodepletion of abundant proteins and multi-lectin affinity chromatography: Application to the analysis of obesity, diabetes, and hypertension diseases [J].

Dayarathna, M. K. Disni R. ;

Hancock, William S. ;

Hincapie, Marina .

JOURNAL OF SEPARATION SCIENCE, 2008, 31 (6-7) :1156-1166

[7] Protein identification using 2D-LC-MS/MS [J].

Delahunty, C ;

Yates, JR .

METHODS, 2005, 35 (03) :248-255

[8] Target-decoy search strategy for increased confidence in large-scale protein identifications by mass spectrometry [J].

Elias, Joshua E. ;

Gygi, Steven P. .

NATURE METHODS, 2007, 4 (03) :207-214

[9] Apoptosis: A review of programmed cell death [J].

Elmore, Susan .

TOXICOLOGIC PATHOLOGY, 2007, 35 (04) :495-516

[10] Uric acid and hypertension in adolescents [J].

Feig, DI .

SEMINARS IN NEPHROLOGY, 2005, 25 (01) :32-38

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