Video observation of protein crystal growth in the advanced protein crystallization facility aboard the space shuttle mission STS-95

被引：10

作者：

Carotenuto, L

Berisio, R

Piccolo, C

Vitagliano, L

Zagari, A

机构：

[1] MARS Ctr, I-80146 Naples, Italy

[2] Univ Naples Federico II, Dipartimento Chim, Ctr Biocristallografia, CNR, I-80134 Naples, Italy

来源：

JOURNAL OF CRYSTAL GROWTH | 2001年 / 232卷 / 1-4期

关键词：

fluid flows; growth from solution; microgravity conditions; proteins;

D O I：

10.1016/S0022-0248(01)01084-3

中图分类号：

O7 [晶体学];

学科分类号：

0702 ; 070205 ; 0703 ; 080501 ;

摘要：

Crystals of polypeptide with sequence (Pro-Pro-Gly)(10) were grown in the Advanced Protein Crystallization Facility during the US Space Shuttle STS-95 Mission. Well diffracting crystals were obtained by dialysis; they provided the highest resolution X-ray data so far collected for a collagen-like triple helical structure (Berisio et al., Acta Crystallogr. D 56 (2000) 55). During the Mission the crystal growth was monitored by a CCD video camera. The image analysis provided information on spatial distribution of crystals, their movements and their growth. The spatial distribution was non-uniform inside the reactors. Limited motions were observed. The linear growth rate indicates that, in some cases, the growth did not cease in the microgravity environment. (C) 2001 Elsevier Science B.V. All rights reserved.

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收藏

页码：481 / 488

页数：8

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