Bright far-red fluorescent protein for whole-body imaging

被引：494

作者：

Shcherbo, Dmitry

Merzlyak, Ekaterina M.

Chepurnykh, Tatiana V.

Fradkov, Arkady F.

Ermakova, Galina V.

Solovieva, Elena A.

Lukyanov, Konstantin A.

Bogdanova, Ekaterina A.

Zaraisky, Andrey G.

Lukyanov, Sergey

Chudakov, Dmitriy M.

机构：

[1] Evrogen JSC, Moscow, Russia

[2] Shemiakin Ovchinnikov Inst Bioorgan Chem, RU-117997 Moscow, Russia

来源：

NATURE METHODS | 2007年 / 4卷 / 09期

关键词：

D O I：

10.1038/NMETH1083

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

For deep imaging of animal tissues, the optical window favorable for light penetration is in near-infrared wavelengths, which requires proteins with emission spectra in the far-red wavelengths. Here we report a far-red fluorescent protein, named Katushka, which is seven-to tenfold brighter compared to the spectrally close HcRed or mPlum, and is characterized by fast maturation as well as a high pH-stability and photostability. These unique characteristics make Katushka the protein of choice for visualization in living tissues. We demonstrate superiority of Katushka for whole-body imaging by direct comparison with other red and far-red fluorescent proteins. We also describe a monomeric version of Katushka, named mKate, which is characterized by high brightness and photostability, and should be an excellent fluorescent label for protein tagging in the far-red part of the spectrum.

引用

收藏

页码：741 / 746

页数：6

相关论文

共 28 条

[11] Bright monomeric red fluorescent protein with an extended fluorescence lifetime [J].

Merzlyak, Ekaterina M. ;

Goedhart, Joachim ;

Shcherbo, Dmitry ;

Bulina, Mariya E. ;

Shcheglov, Aleksandr S. ;

Fradkov, Arkady F. ;

Gaintzeva, Anna ;

Lukyanov, Konstantin A. ;

Lukyanov, Sergey ;

Gadella, Theodorus W. J. ;

Chudakov, Dmitriy M. .

NATURE METHODS, 2007, 4 (07) :555-557

[12] Use of the fluorescent timer DsRED-E5 as reporter to monitor dynamics of gene activity in plants [J].

Mirabella, R ;

Franken, C ;

van der Krogt, GNM ;

Bisseling, T ;

Geurts, R .

PLANT PHYSIOLOGY, 2004, 135 (04) :1879-1887

[13] UPSTREAM SEQUENCES REQUIRED FOR TISSUE-SPECIFIC ACTIVATION OF THE CARDIAC ACTIN GENE IN XENOPUS-LAEVIS EMBRYOS [J].

MOHUN, TJ ;

GARRETT, N ;

GURDON, JB .

EMBO JOURNAL, 1986, 5 (12) :3185-3193

[14]

Nieuwkoop P.D., 1967, Normal Table of Xenopus laevis (Daudin)

[15] Live imaging of fluorescent proteins in chordate embryos: From ascidians to mice [J].

Passamaneck, YJ ;

Di Gregorio, A ;

Papaioannou, VE ;

Hadjantonakis, AK .

MICROSCOPY RESEARCH AND TECHNIQUE, 2006, 69 (03) :160-167

[16] The 2.0-Å crystal structure of eqFP611, a far red fluorescent protein from the sea anemone Entacmaea quadricolor [J].

Petersen, J ;

Wilmann, PG ;

Beddoe, T ;

Oakley, AJ ;

Devenish, RJ ;

Prescott, M ;

Rossjohn, J .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (45) :44626-44631

[17] An improved cyan fluorescent protein variant useful for FRET [J].

Rizzo, MA ;

Springer, GH ;

Granada, B ;

Piston, DW .

NATURE BIOTECHNOLOGY, 2004, 22 (04) :445-449

[18] Visualization of xenotransplanted human rhabdomyosarcoma after transfection with red fluorescent protein [J].

Seitz, Guido ;

Warmann, Steven W. ;

Fuchs, Joerg ;

Mau-Holzmann, Ulrike A. ;

Ruck, Peter ;

Heitmann, Heike ;

Hoffman, Robert M. ;

Mahrt, Jens ;

Mueller, Gerhard A. ;

Wessels, Johannes T. .

JOURNAL OF PEDIATRIC SURGERY, 2006, 41 (08) :1369-1376

[19] Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp red fluorescent protein [J].

Shaner, NC ;

Campbell, RE ;

Steinbach, PA ;

Giepmans, BNG ;

Palmer, AE ;

Tsien, RY .

NATURE BIOTECHNOLOGY, 2004, 22 (12) :1567-1572

[20] Far-red fluorescent proteins evolved from a blue chromoprotein from Actinia equina [J].

Shkrob, MA ;

Yanushevich, YG ;

Chudakov, DM ;

Gurskaya, NG ;

Labas, YA ;

Poponov, SY ;

Mudrik, NN ;

Lukyanov, S ;

Lukyanov, KA .

BIOCHEMICAL JOURNAL, 2005, 392 :649-654