Synthesis and DNA-binding affinities of monomodified berberines

被引：66

作者：

Pang, JY

Qin, Y

Chen, WH ^{[1
]}

Luo, GA

Jiang, ZH

机构：

[1] Hong Kong Baptist Univ, Sch Chinese Med, Kowloon, Hong Kong, Peoples R China

[2] Sun Yat Sen Univ, Sch Chem & Chem Engn, Guangzhou 510275, Guangdong, Peoples R China

[3] Tsinghua Univ, Anal Ctr, Beijing 100084, Peoples R China

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2005年 / 13卷 / 20期

关键词：

berberine; synthesis; DNA-binder; spectrometry;

D O I：

10.1016/j.bmc.2005.05.048

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Four new monomodified berberines have been synthesized in moderate to good yields starting from berberine and fully characterized by HRMS and H-1 NMR. Spectrometric titration and ethidium bromide displacement experiments indicate that these berberine derivatives, especially the one having primary amino group, strongly bind with calf-thymus DNA, presumably via an intercalation mechanism. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：5835 / 5840

页数：6

共 29 条

[1]

Birdsall T. C., 1997, Alternative Medicine Review, V2, P94

[2] Asymmetric intercalation of N1-(acridin-9-ylcarbonyl)spermine at homopurine sites of duplex DNA [J].