Biosynthesis of the angiogenesis inhibitor borrelidin by Streptomyces parvulus Tu4055:: Cluster analysis and assignment of functions

被引：136

作者：

Olano, C

Wilkinson, B

Sánchez, C

Moss, SJ

Sheridan, R

Math, V

Weston, AJ

Braña, AF

Martin, CJ

Oliynyk, M

Méndez, C

Leadlay, PF

Salas, JA ^{[1
]}

机构：

[1] Univ Oviedo, Dept Biol Func, E-33006 Oviedo, Spain

[2] Univ Oviedo, IUOPA, E-33006 Oviedo, Spain

[3] Biot Technol Ltd, Little Chesterford CB10 1XL, Essex, England

[4] Univ Cambridge, Dept Biochem, Cambridge CB2 1GA, England

来源：

CHEMISTRY & BIOLOGY | 2004年 / 11卷 / 01期

关键词：

D O I：

10.1016/j.chembiol.2003.12.018

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The biosynthetic gene cluster for the angiogenesis inhibitor borrelidin has been cloned from Streptomyces parvulus Tu4055. Sequence analysis indicates that the macrolide ring of borrelidin is formed by a modular polyketide synthase (PKS) (borA1-A6), a result that was confirmed by disruption of borA3. The borrelidin PKS; is striking because only seven rather than the nine modules expected for a nonaketide product are encoded by borA1-A6. The starter unit of the PKS has been verified as trans-cyclopentane-1,2-dicarboxylic acid (trans-1,2-CPDA), and the genes involved in its biosynthesis identified. Other genes responsible for biosynthesis of the nitrile moiety, regulation, and self-resistance were also identified.

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收藏

页码：87 / 97

页数：11

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