Visualization of Dynamic Conformational Switching of the G-Quadruplex in a DNA Nanostructure

被引：161

作者：

Sannohe, Yuta ^{[2
]}

Endo, Masayuki ^{[1
,3
]}

Katsuda, Yousuke ^{[2
]}

Hidaka, Kumi ^{[2
]}

Sugiyama, Hiroshi ^{[1
,2
,3
]}

机构：

[1] Kyoto Univ, Inst Integrated Cell Mat Sci iCeMS, Sakyo Ku, Kyoto 6068501, Japan

[2] Kyoto Univ, Dept Chem, Grad Sch Sci, Sakyo Ku, Kyoto 6068502, Japan

[3] Japan Sci & Technol Corp JST, CREST, Chiyoda Ku, Tokyo 1020075, Japan

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2010年 / 132卷 / 46期

关键词：

SEQUENCE FORMS; HUMAN TELOMERE; MUTAGENESIS; STRANDS; MODELS;

D O I：

10.1021/ja1058907

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We herein report the real-time observation of G-quadruplex formation by monitoring the G-quadruplex-induced global change of two duplexes incorporated in a DNA nanoscaffold. The introduced G-rich strands formed an interstrand (3 + 1) G-quadruplex structure in the presence of K+, and the formed four-stranded structure was disrupted by removal of K. These conformational changes were visualized in a nanoscaffold in real-time with fast-scanning atomic force microscopy.

引用

页码：16311 / 16313

页数：3

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