Scarce evidence of yogurt lactic acid bacteria in human feces after daily yogurt consumption by healthy volunteers

被引:61
作者
del Campo, R
Bravo, D
Cantón, R
Ruiz-Garbajosa, P
García-Albiach, R
Montesi-Libois, A
Yuste, FJ
Abraira, V
Baquerol, F
机构
[1] Hosp Univ Ramon & Cajal, Microbiol Serv, Dept Microbiol, Madrid 28034, Spain
[2] Hosp Univ Ramon & Cajal, Microbiol Serv, Dept Prevent Med, Madrid 28034, Spain
[3] Hosp Univ Ramon & Cajal, Microbiol Serv, Dept Biostat, Madrid 28034, Spain
[4] San Pablo CEU Univ, Dept Microbiol, Madrid, Spain
关键词
D O I
10.1128/AEM.71.1.547-549.2005
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In a double-blind prospective study including 114 healthy young volunteers, the presence in human feces of the yogurt organisms Lactobacillus delbrueckii and Streptococcus thermophilus after repeated yogurt consumption (15 days) was analyzed by culture, specific PCR, and DNA hybridization of total fecal DNA. Detection of yogurt lactic acid bacteria in total fecal DNA by bacterial culture and PCR assay was consistently negative. DNA compatible with yogurt bacteria was found by hybridization experiments in only 10 (10.52%) of 96 individuals after consumption of fresh yogurt and in 2 (2.10%) of 96 individuals after consumption of pasteurized yogurt (P = 0.01).
引用
收藏
页码:547 / 549
页数:3
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