Characterization and culture of human embryonic stem cells

被引：309

作者：

Hoffman, LM ^{[1
]}

Carpenter, MK ^{[1
]}

机构：

[1] John P Robarts Res Inst, London, ON N6A 5K8, Canada

来源：

NATURE BIOTECHNOLOGY | 2005年 / 23卷 / 06期

基金：

英国医学研究理事会; 美国国家卫生研究院;

关键词：

D O I：

10.1038/nbt1102

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Human embryonic stem cells have been defined as self-renewing cells that can give rise to many types of cells of the body. How and whether these cells can be manipulated to replace cells in diseased tissues, used to screen drugs and toxins, or studied to better understand normal development, however, depends on knowing more about their fundamental properties. Many different human embryonic stem cell lines-which are pluripotent, proliferate indefinitely in vitro and maintain a normal, euploid karyotype over extended culture-have now been derived, but whether these cell lines are in fact equivalent remains unclear. It will therefore be important to define robust criteria for the assessment of both existing and newly derived cell lines and for the validation of new culture conditions.

引用

页码：699 / 708

页数：10

共 107 条

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