The Hedgehog signaling pathway as a target for anticancer drug discovery

被引:53
作者
Borzillo, GV
Lippa, B
机构
[1] Pfizer Inc, Global Res & Dev, Groton Labs, Canc Biol, Groton, CT 06340 USA
[2] Pfizer Inc, Global Res & Dev, Groton Labs, Canc Chem, Groton, CT 06340 USA
关键词
cancer; hedgehog; patched; smoothened; antagonist; agonist;
D O I
10.2174/1568026053507732
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
The Hedgehog (Hh) signaling pathway directs the development of multiple tissues during embryonic development, and contributes to tissue homeostasis in adults. Deficient Hh signaling results in defective embryogenesis; conversely, excessive Hh signaling is associated with an inherited cancer predisposition syndrome (Gorlin Syndrome), and a growing list of sporadic human cancers. It is now clear that multiple components of "The Hh Pathway" can be altered in tumors. The Hhs are morphogens that signal through effectors that are largely unprecedented in drug discovery, with many key concepts derived from studies in Drosophila melanogaster. However, studies in tumor cell lines have recently identified targets that can be exploited for the discovery of human Hh antagonists, with additional targets likely to emerge as the human pathway is further defined. Here, we review basic aspects of Hh signal transduction, with an emphasis on molecular targets for drug discovery. The use of first-generation Hh antagonists such as cyclopamine will also be discussed; such agents remain invaluable in ongoing efforts to validate drug discovery assays and survey tumor lines for Hh dependence. The various types and frequencies of Hh signaling defects in different human tumors will also be reviewed, as will the status of medicinal chemistry efforts to discover novel Hh antagonists. In section VI, we review assays from the literature that could be utilized to discover new Hh antagonists for the treatment of cancer.
引用
收藏
页码:147 / 157
页数:11
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