Mapping the MinE site involved in interaction with the MinD division site selection protein of Escherichia coli

被引：47

作者：

Ma, LY

King, G

Rothfield, L ^{[1
]}

机构：

[1] Univ Connecticut, Ctr Hlth, Dept Microbiol, Farmington, CT 06032 USA

[2] Univ Connecticut, Ctr Hlth, Dept Biochem, Farmington, CT 06032 USA

来源：

JOURNAL OF BACTERIOLOGY | 2003年 / 185卷 / 16期

关键词：

D O I：

10.1128/JB.185.16.4948-4955.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Interactions between the MinD and MinE proteins are required for proper placement of the Escherichia coli division septum. The site within MinE that is required for interaction with MinD was mapped by studying the effects of site-directed minE mutations on MinD-MinE interactions in yeast two-hybrid and three-hybrid experiments. This confirmed that the MinE N-terminal domain is responsible for the interaction of MinE with MinD. Mutations that interfered with the interaction defined an extended surface on one face of the alpha-helical region of the MinE N-terminal domain, consistent with the idea that the MinE-MinD interaction involves formation of a coiled-coil structure by interaction with a complementary helical surface within MinD.

引用

页码：4948 / 4955

页数：8

共 22 条

[1] A DIVISION INHIBITOR AND A TOPOLOGICAL SPECIFICITY FACTOR CODED FOR BY THE MINICELL LOCUS DETERMINE PROPER PLACEMENT OF THE DIVISION SEPTUM IN ESCHERICHIA-COLI
DEBOER, PAJ
CROSSLEY, RE
ROTHFIELD, LI
[J]. CELL, 1989, 56 (04) : 641 - 649
[2] The MinE ring required for proper placement of the division site is a mobile structure that changes its cellular location during the Escherichia coli division cycle
Fu, XL
Shih, YL
Zhang, Y
Rothfield, LI
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (03) : 980 - 985
[3] Dynamic localization cycle of the cell division regulator MinE in Escherichia coli
Hale, CA
Meinhardt, H
de Boer, PAJ
[J]. EMBO JOURNAL, 2001, 20 (07) : 1563 - 1572
[4] Recruitment of MinC, an inhibitor of Z-ring formation, to the membrane in Escherichia coli: Role of MinD and MinE
Hu, ZL
Saez, C
Lutkenhaus, J
[J]. JOURNAL OF BACTERIOLOGY, 2003, 185 (01) : 196 - 203
[5] Dynamic assembly of MinD on phospholipid vesicles regulated by ATP and MinE
Hu, ZL
Gogol, EP
Lutkenhaus, J
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (10) : 6761 - 6766
[6] Topological regulation of cell division in E-coli: Spatiotemporal oscillation of MinD requires stimulation of its ATPase by MinE and phospholipid
Hu, ZL
Lutkenhaus, J
[J]. MOLECULAR CELL, 2001, 7 (06) : 1337 - 1343
[7] Interaction between FtsZ and inhibitors of cell division
Huang, J
Cao, C
Lutkenhaus, J
[J]. JOURNAL OF BACTERIOLOGY, 1996, 178 (17) : 5080 - 5085
[8] ISHIDATE K, 1986, J BIOL CHEM, V261, P428
[9] The dimerization and topological specificity functions of MinE reside in a structurally autonomous C-terminal domain
King, GF
Rowland, SL
Pan, B
Mackay, JP
Mullen, GP
Rothfield, LI
[J]. MOLECULAR MICROBIOLOGY, 1999, 31 (04) : 1161 - 1169
[10] Structural basis topological specificity function of MinE
King, GF
Shih, YL
Maciejewski, MW
Bains, NPS
Pan, BL
Rowland, SL
Mullen, GP
Rothfield, LI
[J]. NATURE STRUCTURAL BIOLOGY, 2000, 7 (11) : 1013 - 1017

← 1 2 3 →