DNA demethylase is a processive enzyme

被引:36
作者
Cervoni, N [1 ]
Bhattacharya, S [1 ]
Szyf, M [1 ]
机构
[1] McGill Univ, Dept Pharmacol, Montreal, PQ H3G 1Y6, Canada
关键词
D O I
10.1074/jbc.274.13.8363
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
DNA methylation patterns are generated during development by a sequence of methylation and demethylation events. We have recently demonstrated that mammals bear a bona fide demethylase enzyme that removes methyl groups from methylated cytosines. A general genome wide demethylation occurs early in development and in differentiating cell lines. This manuscript tests the hypothesis that the demethylase enzyme is a processive enzyme. Using bisulfite mapping, this report demonstrates that demethylase is a processive enzyme and that the rate limiting step in demethylation is the initiation of demethylation. Initiation of demethylation is determined by the properties of the sequence. Once initiated, demethylation progresses processively. We suggest that these data provide a molecular explanation for global hypomethylation.
引用
收藏
页码:8363 / 8366
页数:4
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