Nanoscale porous silicon waveguide for label-free DNA sensing

被引:153
作者
Rong, Guoguang [1 ]
Najmaie, Ali [2 ,3 ]
Sipe, John E. [2 ,3 ]
Weiss, Sharon M. [1 ]
机构
[1] Vanderbilt Univ, Dept Elect Engn & Comp Sci, Nashville, TN 37212 USA
[2] Univ Toronto, Dept Phys, Toronto, ON M1S 5A7, Canada
[3] Univ Toronto, Inst Opt Sci, Toronto, ON M1S 5A7, Canada
基金
美国国家科学基金会; 加拿大自然科学与工程研究理事会;
关键词
porous silicon; resonant waveguide; DNA biosensor; high sensitivity;
D O I
10.1016/j.bios.2008.01.017
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Porous silicon (Psi) is an excellent material for biosensing due to its large surface area and its capability for molecular size selectivity. In this work, we report the experimental demonstration of a label-free nanoscale Psi resonant waveguide biosensor. The Psi waveguide consists of pores with an average diameter of 20 nm. DNA is attached inside the pores using standard amino-silane and glutaraldehyde chemistry. Molecular binding in the Psi is detected optically based on a shift of the waveguide resonance angle. The magnitude of the resonance shift is directly related to the quantity of biomolecules attached to the pore walls. The Psi waveguide sensor can selectively discriminate between complementary and non-complementary DNA. The advantages of the Psi waveguide biosensor include strong field confinement and a sharp resonance feature, which allow for high sensitivity measurements with a low detection limit. Simulations indicate that the sensor has a detection limit of 50 nM DNA concentration or equivalently, 5 pg/mm(2). (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:1572 / 1576
页数:5
相关论文
共 31 条
[1]  
Amato G, 2000, PHYS STATUS SOLIDI A, V182, P425, DOI 10.1002/1521-396X(200011)182:1<425::AID-PSSA425>3.0.CO
[2]  
2-W
[3]   Novel liquid sensor based on porous silicon optical waveguides [J].
Arrand, HF ;
Benson, TM ;
Loni, A ;
Arens-Fischer, R ;
Kruger, M ;
Thonissen, M ;
Luth, H ;
Kershaw, S .
IEEE PHOTONICS TECHNOLOGY LETTERS, 1998, 10 (10) :1467-1469
[4]   High sensitivity sensors made of perforated waveguides [J].
Awazu, Koichi ;
Rockstuhl, Carsten ;
Fujimaki, Makoto ;
Fukuda, Nobuko ;
Tominaga, Junji ;
Komatsubara, Tetsuro ;
Ikeda, Takahiro ;
Ohki, Yoshimichi .
OPTICS EXPRESS, 2007, 15 (05) :2592-2597
[5]   Identification of gram negative bacteria using nanoscale silicon microcavities [J].
Chan, S ;
Horner, SR ;
Fauchet, PM ;
Miller, BL .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (47) :11797-11798
[6]   Analysis of interaction between chemical agents and porous Si nanostructures using optical sensing properties of infra-red Rugate filters [J].
Chapron, J. ;
Alekseev, S. A. ;
Lysenko, V. ;
Zaitsev, V. N. ;
Barbier, D. .
SENSORS AND ACTUATORS B-CHEMICAL, 2007, 120 (02) :706-711
[7]   A porous silicon optical biosensor: Detection of reversible binding of IgG to a protein A-modified surface [J].
Dancil, KPS ;
Greiner, DP ;
Sailor, MJ .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (34) :7925-7930
[8]   Optical sensors for vapors, liquids, and biological molecules based on porous silicon technology [J].
De Stefano, L ;
Moretti, L ;
Lamberti, A ;
Longo, O ;
Rocchia, M ;
Rossi, AM ;
Arcari, P ;
Rendina, I .
IEEE TRANSACTIONS ON NANOTECHNOLOGY, 2004, 3 (01) :49-54
[9]   DNA optical detection based on porous silicon technology: from biosensors to biochips [J].
De Stefano, Luca ;
Arcari, Paolo ;
Lamberti, Annalisa ;
Sanges, Carmen ;
Rotiroti, Lucia ;
Rea, Ilaria ;
Rendina, Ivo .
SENSORS, 2007, 7 (02) :214-221
[10]   A fiber-optic DNA biosensor microarray for the analysis of gene expression [J].
Ferguson, JA ;
Boles, TC ;
Adams, CP ;
Walt, DR .
NATURE BIOTECHNOLOGY, 1996, 14 (13) :1681-1684