FLT3 expression initiates in fully multipotent mouse hematopoietic progenitor cells

被引：60

作者：

Buza-Vidas, Natalija ^{[1
]}

Woll, Petter ^{[1
]}

Hultquist, Anne ^{[2
]}

Duarte, Sara ^{[1
]}

Lutteropp, Michael ^{[1
]}

Bouriez-Jones, Tiphaine ^{[1
]}

Ferry, Helen ^{[1
]}

Luc, Sidinh ^{[1
,2
]}

Jacobsen, Sten Eirik Waelgaard ^{[1
,3
]}

机构：

[1] Univ Oxford, John Radcliffe Hosp, Weatherall Inst Mol Med, Haematopoiet Stem Cell Lab, Oxford OX3 9DS, England

[2] Lund Univ, Lund Stem Cell Ctr, Hematopoiet Stem Cell Lab, Lund, Sweden

[3] Univ Oxford, John Radcliffe Hosp, Weatherall Inst Mol Med, MRC,Mol Haematol Unit, Oxford OX3 9DS, England

来源：

BLOOD | 2011年 / 118卷 / 06期

基金：

英国医学研究理事会;

关键词：

STEM-CELLS; LINEAGE COMMITMENT; DIFFERENTIATION; IDENTIFICATION; SURVIVAL; LEUKEMIA; LIGAND; MODEL; STAGE; MARKS;

D O I：

10.1182/blood-2010-10-316232

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Lymphoid-primed multipotent progenitors with down-regulated megakaryocyte-erythroid (MkE) potential are restricted to cells with high levels of cell-surface FLT3 expression, whereas HSCs and MkE progenitors lack detectable cell-surface FLT3. These findings are compatible with FLT3 cell-surface expression not being detectable in the fully multipotent stem/progenitor cell compartment in mice. If so, this process could be distinct from human hematopoiesis, in which FLT3 already is expressed in multipotent stem/progenitor cells. The expression pattern of Flt3 (mRNA) and FLT3 (protein) in multipotent progenitors is of considerable relevance for mouse models in which prognostically important Flt3 mutations are expressed under control of the endogenous mouse Flt3 promoter. Herein, we demonstrate that mouse Flt3 expression initiates in fully multipotent progenitors because in addition to lymphoid and granulocyte-monocyte progenitors, FLT3(-) Mk- and E-restricted downstream progenitors are also highly labeled when Flt3-Cre fate mapping is applied. (Blood. 2011;118(6):1544-1548)

引用

页码：1544 / 1548

页数：5

共 26 条

[21]

Srinivas S, 2001, BMC Dev Biol, V1, P4, DOI 10.1186/1471-213X-1-4

[22] Persistent Malignant Stem Cells in del(5q) Myelodysplasia in Remission [J].

Tehranchi, Ramin ;

Woll, Petter S. ;

Anderson, Kristina ;

Buza-Vidas, Natalija ;

Mizukami, Takuo ;

Mead, Adam J. ;

Astrand-Grundstrom, Ingbritt ;

Strombeck, Bodil ;

Horvat, Andrea ;

Ferry, Helen ;

Dhanda, Rakesh Singh ;

Hast, Robert ;

Ryden, Tobias ;

Vyas, Paresh ;

Gohring, Gudrun ;

Schlegelberger, Brigitte ;

Johansson, Bertil ;

Hellstrom-Lindberg, Eva ;

List, Alan ;

Nilsson, Lars ;

Jacobsen, Sten Eirik W. .

NEW ENGLAND JOURNAL OF MEDICINE, 2010, 363 (11) :1025-1037

[23] Adult T-cell progenitors retain myeloid potential [J].

Wada, Haruka ;

Masuda, Kyoko ;

Satoh, Rumi ;

Kakugawa, Kiyokazu ;

Ikawa, Tomokatsu ;

Katsura, Yoshimoto ;

Kawamoto, Hiroshi .

NATURE, 2008, 452 (7188) :768-U10

[24] Interplay of transcription factors in T-cell differentiation and function: the role of Runx [J].

Wong, Won Fen ;

Kohu, Kazuyoshi ;

Chiba, Tomoki ;

Sato, Takehito ;

Satake, Masanobu .

IMMUNOLOGY, 2011, 132 (02) :157-164

[25] Identification of Lin-Sca1+kit+CD34+Flt3- short-term hematopoietic stem cells capable of rapidly reconstituting and rescuing myeloablated transplant recipients [J].

Yang, LP ;

Bryder, D ;

Adolfsson, J ;

Nygren, J ;

Månsson, R ;

Sigvardsson, M ;

Jacobsen, SEW .

BLOOD, 2005, 105 (07) :2717-2723

[26] Early hematopoietic lineage restrictions directed by Ikaros [J].

Yoshida, T ;

Ng, SYM ;

Zuniga-Pflucker, JC ;

Georgopoulos, K .

NATURE IMMUNOLOGY, 2006, 7 (04) :382-391

← 1 2 3 →