Use of degenerate oligonucleotide primed PCR (DOP-PCR) for the genotyping of low-concentration DNA samples

被引：15

作者：

Barbaux, S ^{[1
]}

Poirier, O ^{[1
]}

Cambien, F ^{[1
]}

机构：

[1] INSERM U 525 & SC7, F-75005 Paris, France

来源：

JOURNAL OF MOLECULAR MEDICINE-JMM | 2001年 / 79卷 / 5-6期

关键词：

degenerate oligonucleotide primed amplification (DOP-PCR); DNA; genotyping; single nucleotide polymorphism (SNP); whole genome amplification;

D O I：

10.1007/s001090100214

中图分类号：

Q3 [遗传学];

学科分类号：

071007 [遗传学]; 090102 [作物遗传育种];

摘要：

Degenerate oligonucleotide primed amplification (DOP-PCR) is an efficient method for performing whole genome amplification. We analysed the yield of DNA using this technique starting with various quantities of material. We used DOP-PCR products to genotype single nucleotide polymorphisms and insertion/deletion polymorphisms. DOP-PCR also proved usable for SSCP analysis.

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页码：329 / 332

页数：4

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