Pyrokinin/PBAN radio-receptor assay:: development and application for the characterization of a putative receptor from the pheromone gland of Heliothis peltigera

被引：7

作者：

Altstein, M ^{[1
]}

Ben-Aziz, O

Daniel, S

Zeltser, I

Gilon, C

机构：

[1] Volcani Ctr, Dept Entomol, IL-50250 Bet Dagan, Israel

[2] Hebrew Univ Jerusalem, Dept Organ Chem, IL-91904 Jerusalem, Israel

来源：

PEPTIDES | 2001年 / 22卷 / 09期

基金：

以色列科学基金会;

关键词：

PBAN receptor; PBAN; pheromone glands; Heliothis peltigera; insect neuropeptide; moths; radio receptor assay;

D O I：

10.1016/S0196-9781(01)00479-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A radio-receptor assay (RRA) for the insect pyrokinin/PBAN family has been developed. The development involved examination of the ligand (H-3-tyrosyl-PBAN28-33NH(2))-receptor interaction under various incubation conditions and variations on sex pheromone gland membrane preparation. Application of the RRA for a partial characterization of the putative pyrokinin/PBAN receptor in the pheromone gland of H. peltigera revealed age-dependence of its expression. Pharmacological characterization revealed a high correlation between the binding-affinity to the receptor of various PBAN-derived peptides and their in vivo pheromonotropic bioactivity, and shed light on the interaction of backbone cyclic and linear ([Arg(27),D-Phe(30)]PBAN28-33NH(2)) PBAN antagonists with the receptor. (C) 2001 Elsevier Science Inc. All rights reserved.

引用

页码：1379 / 1389

页数：11

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