Acid-soluble and pepsin-soluble collagens from grass carp (Ctenopharyngodon idella) skin: a comparative study on physicochemical properties

被引：40

作者：

Chen, Ye ^{[1
]}

Ye, Ran ^{[1
]}

Wang, Yan ^{[1
]}

机构：

[1] Tianjin Univ Sci & Technol, Key Lab Food Nutr & Safety, Minist Educ China, Tianjin 300457, Peoples R China

来源：

INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY | 2015年 / 50卷 / 01期

关键词：

Acid-soluble collagen; fish skin; grass carp; pepsin-soluble collagen; PROTEIN HYDROLYSATE; EXTRACTION; OPTIMIZATION; STABILITY; GEL;

D O I：

10.1111/ijfs.12675

中图分类号：

TS2 [食品工业];

学科分类号：

100403 [营养与食品卫生学];

摘要：

In this study, acid-soluble (ASC) and pepsin-soluble (PSC) collagens with triple helical structures were successfully extracted from the skin of grass carp (Ctenopharyngodon idella) by two different extraction approaches. SDS-PAGE pattern revealed that ASC and PSC are type I collagens with typical alpha 1, alpha 2 and beta-chains. In addition, the intensity of v-chain (trimer) in ASC was higher than that of PSC, representing the presence of the high proportion of intra-and intermolecular cross-links of extracted collagens with large molecular weight using the acid method. Differential scanning calorimetry (DSC) results demonstrate that T-d (69.04 degrees C) of ASC was higher than T-d (62.20 degrees C) of PSC. Both ASC and PSC had the highest solubility at acidic pHs or at a low concentration of NaCl (< 2%, w/v). The results of FTIR suggested the ASC and PSC maintained in the helical secondary structure at high degree.

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页码：186 / 193

页数：8

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