Molecular profile of a human monoclonal antibody Fab fragment specific for Epstein-Barr virus gp350/220 antigen

被引：7

作者：

Bugli, F

Bastidas, R

Burton, DR

Williamson, RA

Clementi, M

Burioni, R

机构：

[1] Univ Cattolica Sacro Cuore, Ist Microbiol, I-00168 Rome, Italy

[2] Scripps Res Inst, Dept Immunol, La Jolla, CA 92037 USA

[3] Univ Trieste, Dipartimento Med Sperimentale, Trieste, Italy

[4] Univ Ancona, Fac Med & Chirurg, Ist Microbiol, I-60020 Ancona, Italy

来源：

HUMAN IMMUNOLOGY | 2001年 / 62卷 / 04期

关键词：

phage display; human monoclonal antibodies; combinatorial library; Epstein-Barr virus;

D O I：

10.1016/S0198-8859(01)00216-6

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Experimental evidence indicates Epstein Barr virus (EBV) envelope glycoprotein gp350/220 elicits a potent virus neutralizing response in the infected human host that may play an important role in restricting viral pathogenesis. In this study, we report the molecular cloning in combinatorial phage display vectors, of the IgG1 repertoire of an individual naturally infected with EBV, and describe the recovery and characterization of a monoclonal antibody recognizing gp350/220. A detailed understanding of the human antibody response in EBV infection will identify antibodies of potential use in antiviral prophylaxis and will advance the production of more effective vaccine candidates. (C) American Society for Histocompatibility and Immunogenetics, 2001. Published by Elsevier Science Inc.

引用

收藏

页码：362 / 367

页数：6

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