A comparative study of the purification of betanin

被引：79

作者：

Pires Goncalves, Leticia Christina ^{[1
]}

de Souza Trassi, Marco Aurelio ^{[1
]}

Lopes, Nathana Barbosa ^{[1
]}

Doerr, Felipe Augusto ^{[2
]}

dos Santos, Monica Teixeira ^{[1
]}

Baader, Wilhelm Josef ^{[3
]}

Oliveira, Vani Xavier, Jr. ^{[1
]}

Bastos, Erick Leite ^{[1
]}

机构：

[1] Univ Fed ABC, Ctr Ciencias Nat & Humanas, Santo Andre, SP, Brazil

[2] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, Sao Paulo, Brazil

[3] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, Sao Paulo, Brazil

来源：

FOOD CHEMISTRY | 2012年 / 131卷 / 01期

基金：

巴西圣保罗研究基金会;

关键词：

Betalains; Betacyanins; Betanin; E162; Natural Red 33; PERFORMANCE LIQUID-CHROMATOGRAPHY; IONIZATION MASS-SPECTROMETRY; LATENT POLYPHENOL OXIDASE; TOTAL DISSOLVED SOLIDS; HAIRY ROOT CULTURES; VULGARIS L; RED BEET; ANTIOXIDANT ACTIVITY; DENSITY FUNCTIONALS; BETACYANIN PIGMENTS;

D O I：

10.1016/j.foodchem.2011.08.067

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Betanin is a natural pigment with antioxidant properties used as a food colourant. This work describes the spectrophotometric and chromatographic quantification of betanin (2S/15S) and its epimer isobetanin (2S/15R) in fresh beetroot juice, food-grade beetroot powder and betanin standard diluted in dextrin. Absorption spectra of all three samples were deconvoluted using a mixed three-function model. Food-grade beetroot powder has the largest amount of violet-red impurities, probably formed during processing. The purification of betanin from these complex matrices was carried out by seven different methods. Ion exchange chromatography was the most efficient method for the purification of betanin from all samples; however, fractions contain high amounts of salt. Reversed-phase HPLC as well as reversed-phase column chromatography also produced good results at a much faster rate. The longer retention time of isobetanin when compared to betanin in reversed-phase conditions has been investigated by means of quantum-mechanical methods. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：231 / 238

页数：8

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