Organometallic activation of a fluorogen for templated nucleic acid detection

被引:39
作者
Franzini, Raphael M. [1 ]
Kool, Eric T. [1 ]
机构
[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA
关键词
D O I
10.1021/ol800878b
中图分类号
O62 [有机化学];
学科分类号
070303 ; 081704 ;
摘要
A nucleic acid detection scheme that employs DNA-mediated delivery of an organomercury activator to unmask a fluorophore is described. The approach relies on adjacent hybridization of two oligonucleotide conjugates containing organomercury and caged rhodamine functionalities. Postsynthetic conjugation of amino-modified DNAs enabled efficient preparation of these probes. Complementary DNA templates yielded fluorescence signals arising from metal-assisted rhodamine uncaging.
引用
收藏
页码:2935 / 2938
页数:4
相关论文
共 33 条
[1]   Flow cytometric detection of specific RNAs in native human cells with quenched autoligating FRET probes [J].
Abe, H ;
Kool, ET .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (02) :263-268
[2]   Destabilizing universal linkers for signal amplification in self-ligating probes for RNA [J].
Abe, H ;
Kool, ET .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (43) :13980-13986
[3]   C-5 SUBSTITUTED PYRIMIDINE NUCLEOSIDES .2. SYNTHESIS VIA OLEFIN COUPLING TO ORGANOPALLADIUM INTERMEDIATES DERIVED FROM URIDINE AND 2'-DEOXYURIDINE [J].
BERGSTROM, DE ;
OGAWA, MK .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1978, 100 (26) :8106-8112
[4]   Templated metal catalysis for single nucleotide specific DNA sequence detection [J].
Boll, I ;
Krämer, R ;
Brunner, J ;
Mokhir, A .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (21) :7849-7856
[5]   DNA-templated metal catalysis [J].
Brunner, J ;
Mokhir, A ;
Kraemer, R .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (41) :12410-12411
[6]   Nucleic acid-triggered fluorescent probe activation by the staudinger reaction [J].
Cai, JF ;
Li, XX ;
Yue, X ;
Taylor, JS .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (50) :16324-16325
[7]   CONVERSION OF COVALENTLY MERCURATED NUCLEIC-ACIDS TO TRITIATED AND HALOGENATED DERIVATIVES [J].
DALE, RMK ;
WARD, DC ;
LIVINGSTON, DC ;
MARTIN, E .
NUCLEIC ACIDS RESEARCH, 1975, 2 (06) :915-930
[8]   DIRECT COVALENT MERCURATION OF NUCLEOTIDES AND POLYNUCLEOTIDES [J].
DALE, RMK ;
MARTIN, E ;
LIVINGSTON, DC ;
WARD, DC .
BIOCHEMISTRY, 1975, 14 (11) :2447-2457
[9]   Single nucleotide specific detection of DNA by native chemical ligation of fluorescence labeled PNA-probes [J].
Dose, Christian ;
Seitz, Oliver .
BIOORGANIC & MEDICINAL CHEMISTRY, 2008, 16 (01) :65-77
[10]   DNA-catalyzed transfer of a reporter group [J].
Grossmann, Tom N. ;
Seitz, Oliver .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (49) :15596-15597