New views of cells in 3D: an introduction to electron tomography

被引：319

作者：

McIntosh, R ^{[1
]}

Nicastro, D ^{[1
]}

Mastronarde, D ^{[1
]}

机构：

[1] Univ Colorado, Dept Mol Cellular & Dev Biol, Lab 3D Electron Microscopy Cells, Boulder, CO 80309 USA

来源：

TRENDS IN CELL BIOLOGY | 2005年 / 15卷 / 01期

关键词：

D O I：

10.1016/j.tcb.2004.11.009

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The most important goal of structural cell biology is to elucidate the mechanisms of the processes of life. The structure of a membrane system or fibrous array, the changes in such structures over time or the localizations of enzymes relative to organelle boundaries can often illuminate associated cellular functions. To be of maximal value, structural studies should provide isotropic, 3D information about well-preserved samples at the highest possible resolution. Electron tomography can provide such information about many kinds of cells and organelles.

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页码：43 / 51

页数：9

相关论文

共 75 条

[1] Cryo-electron microscopy of vitreous sections of native biological cells and tissues [J].

Al-Amoudi, A ;

Norlen, LPO ;

Dubochet, J .

JOURNAL OF STRUCTURAL BIOLOGY, 2004, 148 (01) :131-135

[2]

BAILEY B, 2004, MICROSC MICROANAL S1, V10, P90

[3] Electron tomography of molecules and cells [J].

Baumeister, W ;

Grimm, R ;

Walz, J .

TRENDS IN CELL BIOLOGY, 1999, 9 (02) :81-85

[4] Electron tomography: towards visualizing the molecular organization of the cytoplasm [J].

Baumeister, W .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 2002, 12 (05) :679-684

[5] A 3-DIMENSIONAL APPROACH TO MITOTIC CHROMOSOME STRUCTURE - EVIDENCE FOR A COMPLEX HIERARCHICAL ORGANIZATION [J].

BELMONT, AS ;

SEDAT, JW ;

AGARD, DA .

JOURNAL OF CELL BIOLOGY, 1987, 105 (01) :77-92

[6] Specific oligobodies against ERK-2 that recognize both the native and the denatured state of the protein [J].

Bianchini, M ;

Radrizzani, M ;

Brocardo, MG ;

Reyes, GB ;

Solveyra, CG ;

Santa-Coloma, TA .

JOURNAL OF IMMUNOLOGICAL METHODS, 2001, 252 (1-2) :191-197

[7] Systematic evolution of a DNA aptamer binding to rat brain tumor microvessels - Selective targeting of endothelial regulatory protein pigpen [J].

Blank, M ;

Weinschenk, T ;

Priemer, M ;

Schluesener, H .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (19) :16464-16468

[8]

CHIU W, 1986, Journal of Microscopy (Oxford), V141, P385

[9] 3 DIMENSIONAL RECONSTRUCTIONS OF SPHERICAL VIRUSES BY FOURIER SYNTHESIS FROM ELECTRON MICROGRAPHS [J].

CROWTHER, RA ;

AMOS, LA ;

FINCH, JT ;

DEROSIER, DJ ;

KLUG, A .

NATURE, 1970, 226 (5244) :421-&

[10] HIGH-PRESSURE FREEZING FOR THE PRESERVATION OF BIOLOGICAL STRUCTURE - THEORY AND PRACTICE [J].

DAHL, R ;

STAEHELIN, LA .

JOURNAL OF ELECTRON MICROSCOPY TECHNIQUE, 1989, 13 (03) :165-174

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