Vasoactive intestinal polypeptide-like and peptide histidine isoleucine-like proteins excreted/secreted by Nippostrongylus brasiliensis, Nematodirus battus and Ascaridia galli

被引：8

作者：

Foster, N ^{[1
]}

Lee, DL ^{[1
]}

机构：

[1] UNIV LEEDS,DEPT PURE & APPL BIOL,LEEDS LS2 9JT,W YORKSHIRE,ENGLAND

来源：

PARASITOLOGY | 1996年 / 113卷

关键词：

Nematodirus battus; Nippostrongylus brasiliensis; Ascaridia galli; excretions secretions; vasoactive intestinal polypeptide; peptide histidine isoleucine;

D O I：

10.1017/S0031182000082056

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Vasoactive intestinal polypeptide (VIP)-like protein was detected by dot blot analysis in the excretions/secretions (E/S) of Nematodirus battus and Ascaridia galli and was confirmed in the E/S of Nippostrongylus brasiliensis. ELISA analysis showed that N. brasiliensis E/S contained the highest proportion of VIP-like protein (28.04 pmoles/mg of total E/S protein) and A. galli E/S contained the lowest (10.89 pmoles/mg of total E/S protein). Peptide histidine isoleucine (PHI)-like protein was detected by dot blot analysis in the E/S products of N. brasiliensis, N. battus and A. galli. ELISA analysis suggested that A. galli E/S contained the highest proportion of PHI (20.77 nmoles/mg of total E/S protein) and N. battus E/S contained the lowest (0.67 nmoles/mg of total E/S protein). The possible significance of VIP-like and PHi-like substances in the E/S of gastrointestinal nematodes is discussed.

引用

页码：287 / 292

页数：6

共 21 条

[11] THE INFLUENCE OF A CHRONIC SUBCLINICAL INFECTION OF TRICHOSTRONGYLUS-COLUBRIFORMIS ON GASTROINTESTINAL MOTILITY AND DIGESTA FLOW IN SHEEP [J].

GREGORY, PC ;

WENHAM, G ;

POPPI, D ;

COOP, RL ;

MACRAE, JC ;

MILLER, SJ .

PARASITOLOGY, 1985, 91 (OCT) :381-396

[12] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[13]

LEE D L, 1970, Tissue and Cell, V2, P225, DOI 10.1016/S0040-8166(70)80017-9

[14] NUCLEOTIDE-SEQUENCE DIVERGENCE AND FUNCTIONAL CONSTRAINT IN VIP PRECURSOR MESSENGER-RNA EVOLUTION BETWEEN HUMAN AND RAT [J].

NISHIZAWA, M ;

HAYAKAWA, Y ;

YANAIHARA, N ;

OKAMOTO, H .

FEBS LETTERS, 1985, 183 (01) :55-59

[15] NIPPOSTRONGYLUS-BRASILIENSIS - REVIEW OF IMMUNITY AND HOST/PARASITE RELATIONSHIP IN RAT [J].

OGILVIE, BM ;

JONES, VE .

EXPERIMENTAL PARASITOLOGY, 1971, 29 (01) :138-+

[16]

OUCHTERLONY O, 1978, HDB EXPT IMMUNOLOGY, pCH19

[17] CHARACTERIZATION, CLONING AND HOST-PROTECTIVE ACTIVITY OF A 30-KILODALTON GLYCOPROTEIN SECRETED BY THE PARASITIC STAGES OF TRICHOSTRONGYLUS-COLUBRIFORMIS [J].

SAVIN, KW ;

DOPHEIDE, TAA ;

FRENKEL, MJ ;

WAGLAND, BM ;

GRANT, WN ;

WARD, CW .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1990, 41 (02) :167-176

[18] MEASUREMENT OF PROTEIN USING BICINCHONINIC ACID [J].

SMITH, PK ;

KROHN, RI ;

HERMANSON, GT ;

MALLIA, AK ;

GARTNER, FH ;

PROVENZANO, MD ;

FUJIMOTO, EK ;

GOEKE, NM ;

OLSON, BJ ;

KLENK, DC .

ANALYTICAL BIOCHEMISTRY, 1985, 150 (01) :76-85

[19]

TOWBIN H, 1979, P NATL ACAD SCI USA, V76, P43500

[20] COMPARISON OF THE EFFECTS OF NATURAL AND SYNTHETIC-PHI ON GALLBLADDER FLUID TRANSPORT [J].

WOOD, JR ;

BRENNAN, LJ ;

MCLOUGHLIN, TA ;

TATEMOTO, K ;

MUTT, V .

REGULATORY PEPTIDES, 1982, 4 (06) :383-383

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