Lactoferricin B causes depolarization of the cytoplasmic membrane of Escherichia coli ATCC 25922 and fusion of negatively charged liposomes

被引:80
作者
Ulvatne, H [1 ]
Haukland, HH [1 ]
Olsvik, O [1 ]
Vorland, LH [1 ]
机构
[1] Univ Tromso Hosp, Dept Med Microbiol, N-9038 Tromso, Norway
关键词
antimicrobial peptide; lactoferricin; liposome; mode of action;
D O I
10.1016/S0014-5793(01)02233-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Antimicrobial peptides have been extensively studied in order to elucidate their mode of action. Most of these peptides have been shown to exert a bactericidal effect on the cytoplasmic membrane of bacteria. Lactoferricin is an antimicrobial peptide with a net positive charge and an amphipatic structure. In this study we examine the effect of bovine lactoferricin (lactoferricin B; Lfcin B) on bacterial membranes. We show that Lfcin B neither lyses bacteria, nor causes a major leakage from liposomes, Lfcin B depolarizes the membrane of susceptible bacteria, and induces fusion of negatively charged liposomes, Hence, Lfcin B may have additional targets responsible for the antibacterial effect. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:62 / +
页数:5
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