Paparamborde:: a software dedicated to quantitative mapping of biological samples using scanning transmission ion microscopy

被引:5
作者
Devès, G
Michelet-Habchi, C
Ortega, R
机构
[1] Univ Bordeaux 1, CNRS, UMR 5084, F-33175 Gradignan, France
[2] Univ Bordeaux 1, CNRS, IN2P3, UMR 5797,Ctr Etudes Nucl Bordeaux Gradignan, F-33175 Gradignan, France
关键词
scanning transmission ion microscopy; quantitative analysis; biological samples; data analysis;
D O I
10.1016/j.nimb.2005.01.047
中图分类号
TH7 [仪器、仪表];
学科分类号
0804 ; 080401 ; 081102 ;
摘要
Paparamborde, Programme d'Analyse des PArticules RAlenties dans la Mati re de BORDEaux, a software dedicated to the scanning transmission ion microscopy (STIM) analysis of biological samples was developed. The programme includes the reconstruction of the median and/or mean energy loss image of the sample, as well as accurate calculation of sample thickness performed over the whole or a part of the scanned surface. The calculation is based on the conversion of transmitted particle energy into areal density of matter (1.1 mu/cm(2)) and uses SRIM code for the stopping power calculation. A new routine for the calculation of X-ray absorption correction factors (XCF), as needed for PIXE quantification of trace element, has been added in the latest version of Paparamborde, allowing the thickness heterogeneity of sample to be properly taken into account in the calculation of XCF. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:136 / 141
页数:6
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