Isolation and characterisation of T lymphocytes from sural nerve biopsies in patients with Guillain-Barre syndrome and chronic inflammatory demyelinating polyneuropathy

被引:28
作者
BenSmith, A
Gaston, JSH
Barber, PC
Winer, JB
机构
[1] UNIV BIRMINGHAM,SCH MED,DEPT PATHOL,BIRMINGHAM B15 2TT,W MIDLANDS,ENGLAND
[2] UNIV BIRMINGHAM,SCH MED,DEPT NEUROL,BIRMINGHAM B15 2TT,W MIDLANDS,ENGLAND
[3] UNIV BIRMINGHAM,SCH MED,CTR CLIN RES IMMUNOL & SIGNALLING,BIRMINGHAM B15 2TT,W MIDLANDS,ENGLAND
基金
英国惠康基金;
关键词
Guillain-Barre syndrome; chronic inflammatory demyelinating neuropathy; T lymphocytes; sural nerve biopsy;
D O I
10.1136/jnnp.61.4.362
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Objectives-To characterise cultured T lymphocytes from nerve biopsies in patients with Guillain-Barre syndrome and chronic inflammatory demyelinating polyneuropathy (CIDP). Methods-Sural nerve biopsies, obtained from six patients with Guillain-Barre syndrome, four with CIDP, and six controls with other neuropathies, were cultured with 20 U/ml recombinant interleukin-2 (IL-2) for eight weeks. Flow cytometry was used to determine the phenotype of cultured T lymphocytes. Their proliferative responses to a range of bacterial antigens were also examined. Results-T cell lines were established from four of six patients with Guillain-Barre syndrome, one of four with CIDP, one patient with peripheral nerve vasculitis, and none of five controls with noninflammatory neuropathies. One of these T cell lines from a patient with Guillain-Barre syndrome, preceded by Campylobacter jejuni infection, consisted entirely of gamma delta TCR(+) T lymphocytes. The peripheral blood of this patient also contained an increased frequency of gamma delta T cells when stimulated with C jejuni. The nerve derived T cell lines failed to show a proliferative response to bacterial antigens or to a preparation of myelin proteins. Conclusions-A new technique to isolate T cells from nerve biopsies in patients with Guillain-Barre syndrome and CIDP is reported. This technique may prove to be a useful tool in the investigation of the pathogenesis of other inflammatory neuropathies such as peripheral nerve vasculitis. The isolation of a gamma delta TCR(+) nerve T Pathology cell line is of interest because of the possibility that these cells might respond to glycolipid epitopes common to C jejuni and peripheral nerve gangliosides.
引用
收藏
页码:362 / 368
页数:7
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