A multicentric evaluation of IDMS-traceable creatinine enzymatic assays

被引:100
作者
Pieroni, Laurence [2 ]
Delanaye, Pierre [1 ]
Boutten, Anne [3 ]
Bargnoux, Anne-Sophie [4 ]
Rozet, Eric [5 ]
Delatour, Vincent [6 ]
Carlier, Marie-Christine [7 ]
Hanser, Anne-Marie [8 ]
Cavalier, Etienne
Froissart, Marc [9 ]
Cristol, Jean-Paul [4 ]
机构
[1] Univ Liege, Serv Dialyse, CHU Sart Tilman, B-4000 Liege, Belgium
[2] Grp Hosp Pitie Salpetriere, APHP, Paris, France
[3] CHU Bichat, APHP, Paris, France
[4] CHU Lapeyronie, Montpellier, France
[5] Univ Liege, Analyt Chem Lab, CIRM, B-4000 Liege, Belgium
[6] Lab Natl Metrol & Essais, Paris, France
[7] Hop Lyon Sud, Lyon, France
[8] Hosp Civils, Colmar, France
[9] Hop Europeen Georges Pompidou, APHP, Paris, France
关键词
Creatinine; IDMS traceable; Standardization; GLOMERULAR-FILTRATION-RATE; SERUM CREATININE; KIDNEY-DISEASE; RENAL-DISEASE; GC-MS; CALIBRATION; ACCURACY; EQUATION; IMPACT; TIME;
D O I
10.1016/j.cca.2011.07.012
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Chronic kidney disease definition is based on glomerular filtration rate (GFR) estimations which are derived from creatinine-based equations. The accuracy of GFR estimation is thus largely dependent of those of serum creatinine assays. International recommendations highlight the need for traceable creatinine assays. The French Society of Clinical Biochemistry conducted a study for measuring accuracy of creatinine enzymatic methods. This evaluation involved 25 clinical laboratories. Creatinine was measured in serum pools ranging from 35.9 +/- 0.9 mu mol/L to 174.5 +/- 3.1 mu mol/L (IDMS determination) using 12 creatinine enzymatic methods. For all creatinine values greater than 74.4 +/- 1.4 mu mol/L, the bias and imprecision did not exceed 5% and 5.9%, respectively. For the lowest value (35.9 +/- 0.9 mu mol/L), the bias ranged from -1.8 to 9.9% (with one exception). At this level, the imprecision ranged from 1.9 to 7.8%. The true performances of the assays (couples of bias and relative standard deviation), were evaluated using Monte-Carlo simulations. Most of the assays fall within the maximum Total Error of 12% at all concentrations. This study demonstrates substantial improvements in the calibration, traceability and precision of the enzymatic methods, reaching the NKDEP recommendations. Moreover, most of these assays allowed accurate creatinine measurements for creatinine levels lower than 40 mu mol/L. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:2070 / 2075
页数:6
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