Somaclonal variation in plants: causes and detection methods

被引：448

作者：

Bairu, Michael W. ^{[1
]}

Aremu, Adeyemi O. ^{[1
]}

Van Staden, Johannes ^{[1
]}

机构：

[1] Univ KwaZulu Natal Pietermaritzburg, Sch Biol & Conservat Sci, Res Ctr Plant Growth & Dev, ZA-3209 Scottsville, South Africa

来源：

PLANT GROWTH REGULATION | 2011年 / 63卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

Cell and tissue culture; Cell cycle; Chimeras; Chromosome aberration; Cryptic transposable elements; Induced variation; RESTRICTION-FRAGMENT-LENGTH; IN-VITRO CULTURE; CODONOPSIS-LANCEOLATA BENTH; PROTOPLAST-DERIVED PLANTS; POLYMERASE-CHAIN-REACTION; POLYMORPHIC DNA ANALYSIS; TRITICUM-AESTIVUM L; BANANA MUSA SP; TISSUE-CULTURE; SOMATIC EMBRYOGENESIS;

D O I：

10.1007/s10725-010-9554-x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Plant tissue culture has become one of the fundamental tools of plant science research. It is extensively employed in the production, conservation and improvement of plant resources. The presence of somaclonal variation in populations derived from tissue culture is affecting the use of tissue culture negatively and has remained a major problem. Conversely, it is a source of new desirable clones/variants with better agronomic traits. In this review, we summarize the possible causes, detection methods and desirability of variants. Somaclonal variation is one of the most researched and reviewed topics. Hence, we restricted ourselves to outlining various examples which may be used as important references for researchers who intend to identify and/or characterize somaclonal variants while using tissue culture for research and production. Emphasis is placed on the negative effects of somaclonal variation. However, this review also includes examples of some useful variants generated as a result of somaclonal variation.

引用

页码：147 / 173

页数：27

共 303 条

[61]

D'Amato F., 1977, Plant cell, tissue and organ culture. Chapter III. Cytology, cytogenetics and plant breeding., P343

[62] Micropropagated dwarf off-type Cavendish bananas (Musa spp, AAA) show improved tolerance to suboptimal temperatures [J].