Direct enantiomeric resolutions of chiral triazole pesticides by high-perforrnance liquid chromatography

被引:52
作者
Wang, P [1 ]
Jiang, SR [1 ]
Liu, DH [1 ]
Wang, P [1 ]
Zhou, ZQ [1 ]
机构
[1] China Agr Univ, Dept Appl Chem, Beijing 100094, Peoples R China
来源
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2005年 / 62卷 / 03期
基金
中国国家自然科学基金;
关键词
triazole pesticides; chiral resolution; HPLC; CDMPC;
D O I
10.1016/j.jbbm.2004.11.007
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Cellulose-tris (3,5-dimethylphenylcarbamate; CDMPC) was synthesized and coated on amino-propylsilica to prepare chiral stationary phase (CSP). Normal-phase high-performance liquid chromatography (HPLC) methods for the resolutions of five chiral triazole pesticides, diniconazole, tebuconazole, hexaconazole, triadimefon and flutriafol, on the CSP were developed. Several operating parameters such as mobile phase composition, modifier and column temperature were studied for the optimization of the resolutions. Better separations were achieved using 2% isobutanol for diniconazole, 2% ethanol for tebuconazole, 2% iso-propanol for hexaconazole, 1% n-butanol for triadimefon and 2% n-propanol for flutriafol as modifiers in n-hexane at 0 degrees C with the resolution factors (Rs) of 1.62, 1.66, 2.46, 1.68 and 1.98, respectively. Low temperature was better for the resolutions. Validation of the methods included linearity and precision. (c) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:219 / 230
页数:12
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