Introduction of permanently charged groups into PEGA resins leads to improved biotransformations on solid support

被引：11

作者：

Basso, A

Ulijn, RV

Flitsch, SL

Margetts, G

Brazendale, I

Ebert, C

De Martin, L

Linda, P

Verdelli, S

Gardossi, L

机构：

[1] Univ Edinburgh, Sch Chem, Edinburgh EH9 3JJ, Midlothian, Scotland

[2] Univ Trieste, Dipartimento Sci Farmaceut, I-34127 Trieste, Italy

[3] Polymer Labs Ltd, Church Stretton SY6 6AX, Shrops, England

来源：

TETRAHEDRON | 2004年 / 60卷 / 03期

基金：

英国惠康基金; 英国生物技术与生命科学研究理事会;

关键词：

solid phase chemistry; PEGA; charged PEGA; penicillin G amidase; thermolysin;

D O I：

10.1016/j.tet.2003.10.125

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The application of biotransformations in solid phase synthesis is an attractive alternative to chemical methodologies. An important issue that needs to be addressed in this context is accessibility of functional groups within a porous polymer to the biocatalyst. This paper shows that such accessibility can be improved for penicillin G amidase by introducing permanent charges into the polymer. The effect appears to be due to better swelling of the polymer in buffer and to electrostatic interactions between polymer and enzyme. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：589 / 594

页数：6

共 20 条

[1] Organically modified xerogels as supports for solid-phase chemistry [J].