Quantification of isoflavones by capillary zone electrophoresis in soybean seeds:: effects of variety and environment

被引:37
作者
Aussenac, T [1 ]
Lacombe, S [1 ]
Daydé, J [1 ]
机构
[1] ESA Purpan, Lab Agrophysiol, F-31076 Toulouse 03, France
关键词
soybeans; soyfoods; isoflavones; malonyl forms; genistin; daidzin; quantification; capillary zone electrophoresis; environmental factors;
D O I
10.1093/ajcn/68.6.1480S
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
Soybean isoflavones (genistin, daidzin, glycitin, and their malonyl forms and aglucons) are thought to be responsible for the astringent taste of soyfoods. Generally, HPLC with a methanol and water elution gradient is used for isoflavone quantification, but capillary zone electrophoresis (CZE) has been used more recently to separate several flavonoids in plant extracts. We present the results of CZE analysis of isoflavones in soybean extracts. Conditions for separation by using CZE were optimized for analysis of soybean isoflavones. We compared the results of extraction at different temperatures and with different compositions of solvent. Total extraction of isoflavones was not affected by temperature but was affected by composition of the solvent. Malonyl forms of isoflavones were thermally unstable. We analyzed the isoflavone content of different Varieties of soybean seeds sown on different dates. Total isoflavone content varied among different varieties and with sowing dates. Interactions between the variety and the sowing date also affected isoflavone composition. We conclude that the variety of soybean seed and environmental growing conditions, such as sowing date, can contribute to seed quality by reducing its isoflavone content, modifying its isoflavone composition, or both.
引用
收藏
页码:1480S / 1485S
页数:6
相关论文
共 20 条
[1]  
ALBERCREUTZ H, 1992, J STEROID BIOCHEM, V41, P331
[2]  
BARNES S, 1994, ADV EXP MED BIOL, V354, P135
[3]  
CARRAOPANIZZI MC, 1995, BREEDING SCI, V45, P295
[4]   SOYBEAN ISOFLAVONES - EFFECT OF ENVIRONMENT AND VARIETY ON COMPOSITION [J].
ELDRIDGE, AC ;
KWOLEK, WF .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1983, 31 (02) :394-396
[5]   HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY SEPARATION OF SOYBEAN ISOFLAVONES AND THEIR GLUCOSIDES [J].
ELDRIDGE, AC .
JOURNAL OF CHROMATOGRAPHY, 1982, 234 (02) :494-496
[6]   ISOFLAVONE AGLUCONES AND VOLATILE ORGANIC-COMPOUNDS IN SOYBEANS - EFFECTS OF SOAKING TREATMENTS [J].
HA, EYW ;
MORR, CV ;
SEO, A .
JOURNAL OF FOOD SCIENCE, 1992, 57 (02) :414-+
[7]   IMPROVEMENT OF RESOLUTION IN THE CAPILLARY ELECTROPHORETIC SEPARATION OF CATECHOLAMINES BY COMPLEX-FORMATION WITH BORIC-ACID AND CONTROL OF ELECTROOSMOSIS WITH A CATIONIC SURFACTANT [J].
KANETA, T ;
TANAKA, S ;
YOSHIDA, H .
JOURNAL OF CHROMATOGRAPHY, 1991, 538 (02) :385-391
[8]  
KITAMURA K, 1991, JPN J BREED, V41, P651
[9]   MALONYL ISOFLAVONE GLYCOSIDES IN SOYBEAN SEEDS (GLYCINE-MAX MERRILL) [J].
KUDOU, S ;
FLEURY, Y ;
WELTI, D ;
MAGNOLATO, D ;
UCHIDA, T ;
KITAMURA, K ;
OKUBO, K .
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1991, 55 (09) :2227-2233
[10]   DIETARY-EFFECTS ON BREAST-CANCER RISK IN SINGAPORE [J].
LEE, HP ;
GOURLEY, L ;
DUFFY, SW ;
ESTEVE, J ;
LEE, J ;
DAY, NE .
LANCET, 1991, 337 (8751) :1197-1200