Estrogen regulation of TRPM8 expression in breast cancer cells

被引:81
作者
Chodon, Dechen [1 ]
Guilbert, Arnaud [1 ]
Dhennin-Duthille, Isabelle [1 ]
Gautier, Mathieu [1 ]
Telliez, Marie-Sophie [1 ]
Sevestre, Henri [1 ,2 ]
Ouadid-Ahidouch, Halima [1 ]
机构
[1] Univ Picardie Jules Vernes, Lab Physiol Cellulaire & Mol, Fac Sci, JE Canaux Ion Canc Sein 2530, F-80000 Amiens, France
[2] CHU Nord, Serv Anat Pathol, Amiens, France
来源
BMC CANCER | 2010年 / 10卷
关键词
PROSTATE-CANCER; EPITHELIAL-CELLS; RECEPTOR; CHANNELS; PROLIFERATION; ACTIVATION; DIFFERENTIATION; PROTEINS; SURVIVAL; MENTHOL;
D O I
10.1186/1471-2407-10-212
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Background: The calcium-permeable cation channel TRPM8 (melastatin-related transient receptor potential member 8) is over-expressed in several cancers. The present study aimed at investigating the expression, function and potential regulation of TRPM8 channels by ER alpha (estrogen receptor alpha) in breast cancer. Methods: RT-PCR, Western blot, immuno-histochemical, and siRNA techniques were used to investigate TRPM8 expression, its regulation by estrogen receptors, and its expression in breast tissue. To investigate the channel activity in MCF-7 cells, we used the whole cell patch clamp and the calcium imaging techniques. Results: TRPM8 channels are expressed at both mRNA and protein levels in the breast cancer cell line MCF-7. Bath application of the potent TRPM8 agonist Icilin (20 mu M) induced a strong outwardly rectifying current at depolarizing potentials, which is associated with an elevation of cytosolic calcium concentration, consistent with established TRPM8 channel properties. RT-PCR experiments revealed a decrease in TRPM8 mRNA expression following steroid deprivation for 48 and 72 hours. In steroid deprived medium, addition of 17-beta-estradiol (E-2, 10 nM) increased both TRPM8 mRNA expression and the number of cells which respond to Icilin, but failed to affect the Ca2+ entry amplitude. Moreover, silencing ER alpha mRNA expression with small interfering RNA reduced the expression of TRPM8. Immuno-histochemical examination of the expression of TRPM8 channels in human breast tissues revealed an over-expression of TRPM8 in breast adenocarcinomas, which is correlated with estrogen receptor positive (ER+) status of the tumours. Conclusion: Taken together, these results show that TRPM8 channels are expressed and functional in breast cancer and that their expression is regulated by ER alpha.
引用
收藏
页数:8
相关论文
共 31 条
[21]   Estrogens and their receptors in breast cancer progression: a dual role in cancer proliferation and invasion [J].
Platet, N ;
Cathiard, AM ;
Gleizes, M ;
Garcia, M .
CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY, 2004, 51 (01) :55-67
[22]   Ion channels in death and differentiation of prostate cancer cells [J].
Prevarskaya, N. ;
Skryma, R. ;
Bidaux, G. ;
Flourakis, M. ;
Shuba, Y. .
CELL DEATH AND DIFFERENTIATION, 2007, 14 (07) :1295-1304
[23]   TRP channels in cancer [J].
Prevarskaya, Natalia ;
Zhang, Lei ;
Barritt, Greg .
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, 2007, 1772 (08) :937-946
[24]   THE RELATIONSHIP BETWEEN HORMONE RECEPTOR CONTENT AND THE EFFECT OF ADJUVANT TAMOXIFEN IN OPERABLE BREAST-CANCER [J].
RUTQVIST, LE ;
CEDERMARK, B ;
FORNANDER, T ;
GLAS, U ;
JOHANSSON, H ;
NORDENSKJOLD, B ;
ROTSTEIN, S ;
SKOOG, L ;
SOMELL, A ;
THEVE, T ;
WILKING, N ;
ASKERGREN, J ;
HJALMAR, ML .
JOURNAL OF CLINICAL ONCOLOGY, 1989, 7 (10) :1474-1484
[25]   Cooling skin cancer: menthol inhibits melanoma growth. Focus on "TRPM8 activation suppresses cellular viability in human melanoma" [J].
Slominski, Andrzej .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2008, 295 (02) :C293-C295
[26]   Estrogen receptor and breast cancer [J].
Sommer, S ;
Fuqua, SAW .
SEMINARS IN CANCER BIOLOGY, 2001, 11 (05) :339-352
[27]   HUMAN CELL LINE FROM A PLEURAL EFFUSION DERIVED FROM A BREAST CARCINOMA [J].
SOULE, HD ;
VAZQUEZ, J ;
LONG, A ;
ALBERT, S ;
BRENNAN, M .
JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1973, 51 (05) :1409-1416
[28]   Novel role of cold/menthol-sensitive transient receptor potential melastatine family member 8 (TRPM8) in the activation of store-operated channels in LNCaP human prostate cancer epithelial cells [J].
Thebault, S ;
Lemonnier, L ;
Bidaux, G ;
Flourakis, M ;
Bavencoffe, A ;
Gordienko, D ;
Roudbaraki, M ;
Delcourt, P ;
Panchin, Y ;
Shuba, Y ;
Skryma, R ;
Prevarskaya, N .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (47) :39423-39435
[29]  
Tsavaler L, 2001, CANCER RES, V61, P3760
[30]   TRPM8 in prostate cancer cells: a potential diagnostic and prognostic marker with a secretory function? [J].
Zhang, L ;
Barritt, GJ .
ENDOCRINE-RELATED CANCER, 2006, 13 (01) :27-38