Positive-charged solid lipid nanoparticles as paclitaxel drug delivery system in gliohlastoma treatment

被引：73

作者：

Chirio, Daniela ^{[1
]}

Gallarate, Marina ^{[1
]}

Peira, Elena ^{[1
]}

Battaglia, Luigi ^{[1
]}

Muntoni, Elisabetta ^{[1
]}

Riganti, Chiara ^{[2
]}

Biasibetti, Elena ^{[3
]}

Capucchio, Maria Teresa ^{[3
]}

Valazza, Alberto ^{[3
]}

Panciani, Pierpaolo ^{[4
]}

Lanotte, Michele ^{[4
]}

Annovazzi, Laura ^{[5
]}

Caldera, Valentina ^{[5
]}

Mellai, Marta ^{[5
]}

Filice, Gaetano ^{[6
]}

Corona, Silvia ^{[6
]}

Schiffer, Davide ^{[5
]}

机构：

[1] Univ Turin, Dipartimento Sci & Tecnol Farmaco, I-10125 Turin, Italy

[2] Univ Turin, Dipartimento Oncol, Orbassano, TO, Italy

[3] Univ Turin, Dipartimento Sci Vet, Grugliasco, TO, Italy

[4] Univ Turin, Dipartimento Neurosci, I-10125 Turin, Italy

[5] Policlin Monza, Ctr NeuroBioOncol, Vercelli, Italy

[6] Univ Pavia, IRCCS Policlin San Matteo, Dipartimento Malattie Infett, I-27100 Pavia, Italy

来源：

EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS | 2014年 / 88卷 / 03期

关键词：

Paclitaxel; SLN; Coacervation; BBB permeability; Cytotoxicity; Glioblastoma; BLOOD-BRAIN-BARRIER; STEM-LIKE CELLS; IN-VITRO; P-GLYCOPROTEIN; DOXORUBICIN; RESISTANCE; PERMEABILITY; FORMULATION; TRANSPORT; TUMORS;

D O I：

10.1016/j.ejpb.2014.10.017

中图分类号：

R9 [药学];

学科分类号：

100702 [药剂学];

摘要：

Paclitaxel loaded solid lipid nanoparticles (SLN) of behenic acid were prepared with the coacervation technique. Generally, spherical shaped SLN with mean diameters in the range 300-600 nm were obtained. The introduction of charged molecules, such as stearylamine and glycol chitosan into the formulation allowed to obtain positive SLN with Zeta potential in the 8-20 mV range and encapsulation efficiency in the 25-90% range. Blood-brain barrier (BBB) permeability, tested in vitro through hCMEC/D3 cells monolayer, showed a significantly increase in the permeation of Coumarin-6, used as model drug, when vehicled in SLN. Positive-charged SLN do not seem to enhance permeation although stearylamine-positive SLN resulted the best permeable formulation after 24 h. Cytotoxicity studies on NO3 glioblastoma cell line demonstrated the maintenance of cytotoxic activity of all paclitaxel-loaded SLN that was always unmodified or greater compared with free drug. No difference in cytotoxicity was noted between neutral and charged SLN. Co-culture experiments with hCMEC/D3 and different glioblastoma cells evidenced that, when delivered in SLN, paclitaxel increased its cytotoxicity towards glioblastoma cells. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：746 / 758

页数：13

共 53 条

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