Continuous fluorometric assays for acetylcholinesterase activity and inhibition with conjugated polyelectrolytes

被引：153

作者：

Feng, Fude ^{[1
]}

Tang, Yanli ^{[1
]}

Wang, Shu ^{[1
]}

Li, Yuliang ^{[1
]}

Zhu, Daoben ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Chem, Natl Lab Mol Sci, Key Lab Organ Solids, Beijing 100080, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 41期

关键词：

biosensors; conjugated polymers; drug screening; enzyme catalysis; fluorescent probes;

D O I：

10.1002/anie.200701724

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Quick but sensitive: A super-quenched fluorogenic complex of a cationic acetylcholine (ACh) derivative with an energy-acceptor tag and an anionic water-soluble conjugated polymer forms the basis of a highly effective fluorescence turn-on assay for studying the enzyme kinetics and inhibition of acetylcholinesterase (AChE; see schematic representation of the assay). FRET = fluorescence resonant energy transfer. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

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收藏

页码：7882 / 7886

页数：5

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