Cationic conjugated polymer/DNA complexes for amplified fluorescence assays of nucleases and methyltransferases

被引：100

作者：

Feng, Fude ^{[1
]}

Tang, Yanli ^{[1
]}

He, Fang ^{[1
]}

Yu, Minghui ^{[1
]}

Duan, Xinrui ^{[1
]}

Wang, Shu ^{[1
]}

Li, Yuliang ^{[1
]}

Zhu, Daoben ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Key Lab Organ Solids, Beijing 100080, Peoples R China

来源：

ADVANCED MATERIALS | 2007年 / 19卷 / 21期

关键词：

D O I：

10.1002/adma.200700165

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A new method is developed for sensitive, homogeneous, and convenient assays of nucleases and methyltransferases using complexes of cationic conjugated polymers with DNA containing a fluorescein tag at the 5'-terminus (DNA-F1). The cleavage of DNA by nucleases can be monitored by fluorescence spectra by observing conjugated-polymer or fluorescein emission changes in aqueous solutions.

引用

收藏

页码：3490 / +

页数：7

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