Simultaneous Determination of Deoxynivalenol and Nivalenol in Traditional Chinese Medicine by SPE and LC

被引：13

作者：

Yue, Yan-Tao ^{[1
,2
]}

Zhang, Xiao-Fei ^{[1
,2
]}

Yang, Mei-Hua ^{[1
]}

Zhen Ou-Yang ^{[2
]}

Liu, Hong-Bing ^{[3
]}

机构：

[1] Peking Union Med Coll, Chinese Acad Med Sci, Inst Med Plant Dev, Beijing 100193, Peoples R China

[2] Jiangsu Univ, Sch Pharm, Zhenjiang 212013, Peoples R China

[3] Beijing Rapidbio Sci Co Ltd, Beijing 100073, Peoples R China

来源：

CHROMATOGRAPHIA | 2010年 / 72卷 / 5-6期

关键词：

Column liquid chromatography; UV detection; Deoxynivalenol purification column; Traditional Chinese medicine; Deoxynivalenol and nivalenol; CHROMATOGRAPHY-MASS-SPECTROMETRY; TRICHOTHECENE MYCOTOXINS; LIQUID-CHROMATOGRAPHY; ANIMAL FEED; CEREALS; IONIZATION; CLEANUP; GRAINS; HEALTH; BEER;

D O I：

10.1365/s10337-010-1679-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A liquid chromatography method for simultaneous determination of deoxynivalenol (DON) and nivalenol (NIV) in traditional Chinese medicine (TCM) is described for the first time. Different clean-up columns and extraction solvents have been optimized. The DON purification column was chosen as the best. The detection limits for DON and NIV were 62.5 and 50.0 mu g kg(-1), respectively. Recoveries from different TCMs spiked with DON and NIV at levels ranging from 0.5 to 10 mg kg(-1) were 82.8-99.9% and 80.8-100.3%, respectively. None of the 30 commercially available TCM samples analyzed, were found to contain any detectable amounts of DON and NIV.

引用

页码：551 / 555

页数：5

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