Simultaneous Determination of Deoxynivalenol and Nivalenol in Traditional Chinese Medicine by SPE and LC

被引:13
作者
Yue, Yan-Tao [1 ,2 ]
Zhang, Xiao-Fei [1 ,2 ]
Yang, Mei-Hua [1 ]
Zhen Ou-Yang [2 ]
Liu, Hong-Bing [3 ]
机构
[1] Peking Union Med Coll, Chinese Acad Med Sci, Inst Med Plant Dev, Beijing 100193, Peoples R China
[2] Jiangsu Univ, Sch Pharm, Zhenjiang 212013, Peoples R China
[3] Beijing Rapidbio Sci Co Ltd, Beijing 100073, Peoples R China
关键词
Column liquid chromatography; UV detection; Deoxynivalenol purification column; Traditional Chinese medicine; Deoxynivalenol and nivalenol; CHROMATOGRAPHY-MASS-SPECTROMETRY; TRICHOTHECENE MYCOTOXINS; LIQUID-CHROMATOGRAPHY; ANIMAL FEED; CEREALS; IONIZATION; CLEANUP; GRAINS; HEALTH; BEER;
D O I
10.1365/s10337-010-1679-6
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A liquid chromatography method for simultaneous determination of deoxynivalenol (DON) and nivalenol (NIV) in traditional Chinese medicine (TCM) is described for the first time. Different clean-up columns and extraction solvents have been optimized. The DON purification column was chosen as the best. The detection limits for DON and NIV were 62.5 and 50.0 mu g kg(-1), respectively. Recoveries from different TCMs spiked with DON and NIV at levels ranging from 0.5 to 10 mg kg(-1) were 82.8-99.9% and 80.8-100.3%, respectively. None of the 30 commercially available TCM samples analyzed, were found to contain any detectable amounts of DON and NIV.
引用
收藏
页码:551 / 555
页数:5
相关论文
共 23 条
[1]   Quantification of deoxynivalenol in wheat using an immunoaffinity column and liquid chromatography [J].
Cahill, LM ;
Kruger, SC ;
McAlice, BT ;
Ramsey, CS ;
Prioli, R ;
Kohn, B .
JOURNAL OF CHROMATOGRAPHY A, 1999, 859 (01) :23-28
[2]  
Dall'Asta C, 2004, J CHROMATOGR A, V1054, P389, DOI [10.1016/j.chroma.2004.07.081, 10.1016/S0021-9673(04)01285-3]
[3]   Toxicological evaluation of trichothecenes in animal feed [J].
Eriksen, GS ;
Pettersson, H .
ANIMAL FEED SCIENCE AND TECHNOLOGY, 2004, 114 (1-4) :205-239
[4]   First survey of deoxynivalenol occurrence in crops in Serbia [J].
Jajic, Igor ;
Juric, Verica ;
Abramovic, Bijana .
FOOD CONTROL, 2008, 19 (06) :545-550
[5]   Analysis of the Fusarium mycotoxins fusaproliferin and trichothecenes in grains using gas chromatography-mass spectrometry [J].
Jestoi, M ;
Ritieni, A ;
Rizzo, A .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2004, 52 (06) :1464-1469
[6]   Determination of trichothecenes in cereals [J].
Kotal, F ;
Holadová, K ;
Hajslová, J ;
Poustka, J ;
Radová, Z .
JOURNAL OF CHROMATOGRAPHY A, 1999, 830 (01) :219-225
[7]   The state-of-the-art in the analysis of type-A and -B trichothecene mycotoxins in cereals [J].
Krska, R ;
Baumgartner, S ;
Josephs, R .
FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 2001, 371 (03) :285-299
[8]   Occurrence of trichothecene mycotoxins in Brazilian corn-based food products [J].
Milanez, TV ;
Valente-Soares, LM ;
Baptista, GG .
FOOD CONTROL, 2006, 17 (04) :293-298
[10]   Occurrence of deoxynivalenol (vomitoxin) in beer in Turkey detected by HPLC [J].
Omurtag, Gulden Z. ;
Beyoglu, Diren .
FOOD CONTROL, 2007, 18 (02) :163-166