Reprogramming of EBV-immortalized B-lymphocyte cell lines into induced pluripotent stem cells

被引：66

作者：

Choi, Su Mi ^{[1
]}

Liu, Hua ^{[1
]}

Chaudhari, Pooja ^{[1
]}

Kim, Yonghak ^{[1
]}

Cheng, Linzhao ^{[2
,3
]}

Feng, Jian ^{[4
]}

Sharkis, Saul ^{[1
]}

Ye, Zhaohui ^{[2
,3
]}

Jang, Yoon-Young ^{[1
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Stem Cell Biol Lab, Sidney Kimmel Comprehens Canc Ctr, Baltimore, MD 21231 USA

[2] Johns Hopkins Univ, Sch Med, Inst Cell Engn, Div Hematol, Baltimore, MD 21231 USA

[3] Johns Hopkins Univ, Sch Med, Inst Cell Engn, Stem Cell Program, Baltimore, MD 21231 USA

[4] SUNY Buffalo, Dept Physiol & Biophys, Buffalo, NY 14260 USA

来源：

BLOOD | 2011年 / 118卷 / 07期

基金：

美国国家卫生研究院;

关键词：

BLOOD-CELLS; EXPRESSION; GENERATION; TRANSFORMATION; DERIVATION;

D O I：

10.1182/blood-2011-03-340620

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

EBV-immortalized B lymphocyte cell lines have been widely banked for studying a variety of diseases, including rare genetic disorders. These cell lines represent an important resource for disease modeling with the induced pluripotent stem cell (iPSC) technology. Here we report the generation of iPSCs from EBV-immortalized B-cell lines derived from multiple inherited disease patients via a nonviral method. The reprogramming method for the EBV cell lines involves a distinct protocol compared with that of patient fibroblasts. The B-cell line-derived iPSCs expressed pluripotency markers, retained the inherited mutation and the parental V(D)J rearrangement profile, and differentiated into all 3 germ layer cell types. There was no integration of the reprogramming-related transgenes or the EBV-associated genes in these iPSCs. The ability to reprogram the widely banked patient B-cell lines will offer an unprecedented opportunity to generate human disease models and provide novel drug therapies. (Blood. 2011; 118(7): 1801-1805)

引用

页码：1801 / 1805

页数：5

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