Antibody detection of SARS-CoV spike and nucleocapsid protein

被引：61

作者：

Chang, MS

Lu, YT

Ho, ST

Wu, CC

Wei, TY

Chen, CJ

Hsu, YT

Chu, PC

Chen, CH

Chu, JM

Jan, YL

Hung, CC

Fan, CC

Yang, YC ^{[1
]}

机构：

[1] Mackay Mem Hosp, Dept Med Res, Taipei, Taiwan

[2] Natl Taipai Univ Technol, Taipei, Taiwan

[3] Taipei Med Univ, Taipei, Taiwan

[4] Mackay Mem Hosp, Dept Lab Med, Taipei, Taiwan

[5] Mackay Mem Hosp, Dept Pathol, Taipei, Taiwan

[6] Mackay Mem Hosp, Dept Obstet & Gynecol, Taipei, Taiwan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 314卷 / 04期

关键词：

SARS; spike; nucleocapsid;

D O I：

10.1016/j.bbrc.2003.12.195

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Early detection and identification of SARS-CoV-infected patients and actions to prevent transmission are absolutely critical to prevent another SARS outbreak. Antibodies that specifically recognize the SARS-CoV spike and nucleocapsid proteins may provide a rapid screening method to allow accurate identification and isolation of patients with the virus early in their infection. For this reason, we raised peptide-induced polyclonal antibodies against SARS-CoV spike protein and polyclonal antibodies against SARS-CoV nucleocapsid protein using 6x His nucleocapsid recombinant protein. Western blot analysis and immunofluorescent staining showed that these antibodies specifically recognized SARS-CoV. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：931 / 936

页数：6

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