Gene gun-mediate DNA vaccination against foot-and-mouth disease virus

被引：52

作者：

Benvenisti, L

Rogel, A

Kuznetzova, L

Bujanover, S

Becker, Y

Stram, Y

机构：

[1] Kimron Vet Inst, Div Virol, IL-50250 Bet Dagan, Israel

[2] Hebrew Univ Jerusalem, Hadassah Med Sch, Dept Mol Virol, IL-91905 Jerusalem, Israel

来源：

VACCINE | 2001年 / 19卷 / 28-29期

关键词：

long and accurate-reverse transcriptase-polymerase chain reaction; foot-and-mouth disease virus; vaccine;

D O I：

10.1016/S0264-410X(01)00125-6

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Foot-and-mouth disease (FMD) is one of the most dangerous diseases of cloven-hoofed animals and is a constant threat in the Middle-East and other regions throughout the world despite intensive Vaccination programs. In this work, we describe the ability of FMDV expression constructs to protect pigs from FMDV challenge when used as a vaccine. The construct consists of encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES), the entire P1 and 2A together with 3CD sequences, all in the same reading frame. Another plasmid that was tested, carries the serotype O1 (G) VPI, Asia1 VP1 and O1 (G) 3C. Between each of the genes the 3C cleavage sequences were inserted. All constructs carried the cytomegalo virus (CMV) promoter. Using immunofluorescent and immunoblot techniques, we could show the expression and processing of viral proteins. Following the application of FMDV expression constructs into pigs skin by 'Gene Gun', pigs were partially protected from FMDV challenge. (C) 2001 Published by Elsevier Science Ltd.

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页码：3885 / 3895

页数：11

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