Water-based ligand screening for paramagnetic metalloproteins

被引：17

作者：

Bertini, Ivano ^{[1
,2
]}

Fragai, Marco ^{[1
,3
]}

Luchinat, Claudio ^{[1
,3
]}

Talluri, Eleonora ^{[1
]}

机构：

[1] Univ Florence, Magnet Resonance Ctr, I-50019 Sesto Fiorentino, Italy

[2] Univ Florence, Dept Chem, I-50019 Sesto Fiorentino, Italy

[3] Univ Florence, Dept Agr Biotechnol, I-50144 Florence, Italy

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 24期

关键词：

drug design; metalloenzymes; metalloproteins; NMR spectroscopy; proton relaxation;

D O I：

10.1002/anie.200800327

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The relaxation rate of water proton nuclei can be used to screen ligands (L; see picture) directed to the metal binding site in paramagnetic metalloproteins by NMR spectroscopy. The very high concentration of water protons provides an enormous sensitivity and permits the use of unprecedentedly low amounts of protein. (Figure Presented). © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

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页码：4533 / 4537

页数：5

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