Rapid determination of viral RNA sequences in mosquitoes collected in the field

被引：32

作者：

Kihara, Yuki ^{[1
]}

Satho, Tomomitsu ^{[1
]}

Eshita, Yuki ^{[4
]}

Sakai, Kouji ^{[2
]}

Kotaki, Akira ^{[2
]}

Takasaki, Tomohiko ^{[2
]}

Rongsriyam, Yupha ^{[5
]}

Komalamisra, Narurnon ^{[5
]}

Srisawat, Raweewan ^{[5
]}

Lapcharoen, Parichat ^{[5
]}

Sumroiphon, Suchada ^{[5
]}

Iwanaga, Shiroh ^{[6
]}

Ushijima, Hiroshi ^{[7
]}

Endoh, Daiji ^{[3
]}

Miyata, Takeshi ^{[1
]}

Sakata, Akira ^{[1
]}

Kashige, Nobuhiro ^{[1
]}

Miake, Fumio ^{[1
]}

Fukushi, Shuetsu ^{[2
]}

Saijo, Masayuki ^{[2
]}

Kurane, Ichiro ^{[2
]}

Morikawa, Shigeru ^{[2
]}

Mizutani, Tetsuya ^{[2
]}

机构：

[1] Fukuoka Univ, Fac Pharmaceut Sci, Jonan Ku, Fukuoka 8140180, Japan

[2] Natl Inst Infect Dis, Tokyo 2080011, Japan

[3] Rakuno Gakuen Univ, Sch Vet Med, Ebetsu, Hokkaido, Japan

[4] Oita Univ, Fac Med, Dept Infect Dis, Oita 8795593, Japan

[5] Mahidol Univ, Fac Trop Med, Dept Med Entomol, Bangkok 10400, Thailand

[6] Kobe Univ, Fac Agr, Dept Biofunctional Chem, Nada Ku, Kobe, Hyogo 6578501, Japan

[7] Univ Tokyo, Grad Sch Med, Dept Dev Med Sci, Tokyo 1130033, Japan

来源：

JOURNAL OF VIROLOGICAL METHODS | 2007年 / 146卷 / 1-2期

基金：

日本学术振兴会;

关键词：

rapid determination; dengue virus type 4; cell fusing agent virus; Aedes aegypti;

D O I：

10.1016/j.jviromet.2007.07.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for rapid determination of viral RNA sequences (RDV) was applied to homogenates of Aedes aegypti collected in Thailand in an area in which dengue fever (dengue hemorrhagic fever) is endemic, using the mosquito cell line C6/36. Nucleic acid sequences of dengue virus type 4 and cell fusing agent virus were detected. This RDV method has the potential to become a standard method for detection of both known and newly emerging, unknown mosquito-borne viruses. (c) 2007 Published by Elsevier B.V.

引用

页码：372 / 374

页数：3

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