Phylogenetic and expression analysis of the NPR1-like gene family from Persea americana (Mill.)

被引：34

作者：

Backer, Robert ^{[1
,2
]}

Mahomed, Waheed ^{[1
,2
]}

Reeksting, Bianca J. ^{[1
,2
]}

Engelbrecht, Juanita ^{[1
,2
]}

Ibarra-Laciette, Enrique ^{[3
,4
]}

van den Berg, Noelani ^{[1
,2
]}

机构：

[1] Univ Pretoria, Forestry & Agr Biotechnol Inst, ZA-0002 Pretoria, South Africa

[2] Univ Pretoria, Dept Genet, Fruit Tree Biotechnol Program, Plant Sci Complex Room 5-17, ZA-0002 Pretoria, South Africa

[3] Inst Politecn Nacl, Ctr Invest & Estudios Avanzados, Lab Nacl Genom Biodiversidad Langebio, Unidad Genom Avanzada, Irapuato, Mexico

[4] Inst Ecol AC, Red Estudios Mol Avanzados, Xalapa, Veracruz, Mexico

来源：

FRONTIERS IN PLANT SCIENCE | 2015年 / 6卷

基金：

新加坡国家研究基金会;

关键词：

avocado; Phytophthora cinnamomi; NPR1; expression analysis; salicylic acid; jasmonic acid; pathogenesis-related; SYSTEMIC ACQUIRED-RESISTANCE; REAL-TIME PCR; SALICYLIC-ACID; DISEASE RESISTANCE; PHYTOPHTHORA-CINNAMOMI; TRANSCRIPTION FACTORS; ARABIDOPSIS NPR1; PLANT-DISEASE; PSEUDOMONAS-SYRINGAE; NEGATIVE REGULATOR;

D O I：

10.3389/fpls.2015.00300

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The NONEXPRESSOR OF PATHOGENESIS-RELATED GENES1 (NPR1) forms an integral part of the salicylic acid (SA) pathway in plants and is involved in cross-talk between the SA and jasmonic acid/ethylene (JA/ET) pathways. Therefore, NPR1 is essential to the effective response of plants to pathogens. Avocado (Persea americana) is a commercially important crop worldwide. Significant losses in production result from Phytophthora root rot, caused by the hemibiotroph, Phytophthora cinnamomi. This oomycete infects the feeder roots of avocado trees leading to an overall decline in health and eventual death. The interaction between avocado and P. cinnamomi is poorly understood and as such limited control strategies exist. Thus uncovering the role of NPR1 in avocado could provide novel insights into the avocado - P. cinnamomi interaction. A total of five NPR1-like sequences were identified. These sequences were annotated using FGENESH and a maximumlikelihood tree was constructed using 34 NPR1-like protein sequences from other plant species. The conserved protein domains and functional motifs of these sequences were predicted. Reverse transcription quantitative PCR was used to analyze the expression of the five NPR1-like sequences in the roots of avocado after treatment with salicylic and jasmonic acid, P. cinnamomi infection, across different tissues and in P. cinnamomi infected tolerant and susceptible rootstocks. Of the five NPR1-like sequences three have strong support for a defensive role while two are most likely involved in development. Significant differences in the expression profiles of these five NPR1-like genes were observed, assisting in functional classification. Understanding the interaction of avocado and P. cinnamomi is essential to developing new control strategies. This work enables further classification of these genes by means of functional annotation and is a crucial step in understanding the role of NPR1 during P. cinnamomi infection.

引用

页数：15

共 87 条

[11] Overexpressing MhNPR1 in transgenic Fuji apples enhances resistance to apple powdery mildew [J].