Sesquiterpenes from Baizhu Stimulate Glucose Uptake by Activating AMPK and PI3K

被引:88
作者
Chao, Chien-Liang [1 ]
Huang, Hui-Chi [4 ]
Lin, Hang-Ching [2 ]
Chang, Tsu-Chung [1 ,3 ,5 ,6 ]
Chang, Wen-Liang [1 ,2 ]
机构
[1] Natl Def Med Ctr, Grad Inst Life Sci, Taipei 114, Taiwan
[2] Natl Def Med Ctr, Sch Pharm, Taipei 114, Taiwan
[3] Natl Def Med Ctr, Dept Biochem, POB 90048, Taipei 114, Taiwan
[4] China Med Univ, Dept Chinese Pharmaceut Sci & Chinese Med Resourc, Taichung 404, Taiwan
[5] Asia Univ, Dept Biotechnol, Taichung, Taiwan
[6] China Med Univ, China Med Univ Hosp, Taichung 402, Taiwan
来源
AMERICAN JOURNAL OF CHINESE MEDICINE | 2016年 / 44卷 / 05期
关键词
Baizhu; Sesquiterpenes; GLUT4; C2C12; Myotubes; AMP-activated Protein Kinase; PI3K; TRANSPORTER; ADIPOCYTES;
D O I
10.1142/S0192415X16500531
中图分类号
R [医药、卫生];
学科分类号
100218 [急诊医学];
摘要
Baizhu, the dried rhizome of Atractylodes Macrocephala Koidz (Compositae), is one of the most important traditional Chinese herbal medicines. Baizhu is generally used to treat digestive disorders and diabetes in Asian countries. This study investigates the activity of two sesquiterpenes isolated from Baizhu, atractylenolide I (AT-I) and atractylenolide II (AT-II), for their effects on glucose uptake in mouse skeletal muscle C2C12 cells, and the corresponding mechanism. These compounds show a significant stimulatory effect on glucose uptake in C2C12 myotubes. Both AT-I and AT-II significantly increased GLUT4 but not GLUT1 protein levels, and promoted GLUT4 translocation to the plasma membrane. The increased glucose uptake induced by these compounds is associated with activation of AMP-activated protein kinase (AMPK) and PI3K/Akt pathways in these cells. Further studies have indicated that AT-I and AT-II ameliorate TNF-alpha-induced insulin resistance in C2C12 myotubes. In summary, our findings highlight the insulin mimetic activity of Baizhu in myotubes, and provide insights into the action mechanism underlying these effects. Our findings may also prove highly relevant to the development of novel therapeutic applications for these compounds.
引用
收藏
页码:963 / 979
页数:17
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