A new method for the production of L-lyxose from ribitol using microbial and enzymatic reactions

被引:26
作者
Bhuiyan, SH [1 ]
Ahmed, Z [1 ]
Utamura, M [1 ]
Izumori, K [1 ]
机构
[1] Kagawa Univ, Fac Agr, Dept Biochem & Food Sci, Miki, Kagawa 7610795, Japan
来源
JOURNAL OF FERMENTATION AND BIOENGINEERING | 1998年 / 86卷 / 05期
关键词
Acetobacter aceti IFO 3281; L-rhamnose isomerase; D-tagatose; 3-epimerase; ribitol; L-ribulose; L-lyxose;
D O I
10.1016/S0922-338X(98)80163-3
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
L-Lyxose was prepared from ribitol by a. new method comprising a potent microbial oxidation reaction to convert ribitol to L-ribulose, epimerization of the L-ribulose to L-xylulose, and isomerization of the L-xylulose to produce L-lyxose. The complete transformation of ribitol to L-ribulose was achieved using washed cells of Acetobacter aceti IFO 3281 at high substrate concentrations ranging from 5-20%. The L-ribulose produced was then used as the substrate for the production of L-lyxose using immobilized L-rhamnose isomerase (L-RI) of Pseudomonas sp. strain LL172 and immobilized D-tagatose 3-epimerase (D-TE) of recombinant Escherichia coli JM 105. At equilibrium, the yield of L-lyxose from L-ribulose was determined to be about 60%, and the product could be isolated easily from the reaction mixture after degradation of ketoses using Pseudomonas sp. 172a. Following various product purification steps, about 5.0 g L-lyxose crystals were recovered from 10.0 g ribitol in a flask reaction. The crystallized product was finally identified by HPLC, IR spectrum, NMR, and optical rotation measurements.
引用
收藏
页码:513 / 516
页数:4
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