Differential hormonal regulation of tryptophan hydroxylase-2 mRNA in the murine dorsal raphe nucleus

被引:50
作者
Clark, JA [1 ]
Pai, LY [1 ]
Flick, RB [1 ]
Rohrer, SP [1 ]
机构
[1] Merck & Co Inc, Merck Res Labs, Dept Mol Endocrinol, Rahway, NJ 07065 USA
关键词
mouse; tryptophan hydroxylase; raphe; glucocorticoid; estrogen; nnRNA;
D O I
10.1016/j.biopsych.2005.01.013
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Background. Recently a novel tryptophan hydroxylase isoform (TPH2) was identified and shown to be highly expressed in the central nervous system (CNS). Hormonal effects on TPH2 mRNA expression in the rodent dorsal raphe nucleus (DRN) are unknown. Methods. In situ hybridization histochemistry and real-time reverse transcriptase-polymerase chain reaction (RT-PCR) were used to assess the effects of dexamethasone or estradiol on TPH2 mRNA levels in the DRN of C57/Bl6 mice. Results: Dexamethasone reduced TPH2 mRNA levels in the DRN of both ovx female and intact male mice. Reduction of TPH2 mRNA in the DRAT was blocked by co-administration of mifepristone. Estradiol had no detectable effect on TPH2 mRNA levels in the DRN Conclusions. TPH2 mRNA is regulated by glucocorticoids but not estradiol in the mouse DRN. Glucocorticoid-mediated reduction of TPH2 message may have relevance to the etiology of major depression, psychotic major depression in particular, where elevated glucocorticoids are one hallmark of the disease.
引用
收藏
页码:943 / 946
页数:4
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