B7-H3 regulates the development of experimental allergic conjunctivitis in mice

被引:53
作者
Fukushima, Atsuki
Sumi, Tamaki
Fukuda, Ken
Kumagai, Naoki
Nishida, Teruo
Yamazaki, Tomohide
Akiba, Hisaya
Okumura, Ko
Yagita, Hideo
Ueno, Hisayuki
机构
[1] Kochi Med Sch, Dept Ophthalmol & Visual Sci, Nankoku, Kochi 7838505, Japan
[2] Yamaguchi Univ, Sch Med, Dept Ophthalmol, Ube, Yamaguchi, Japan
[3] Juntendo Univ, Dept Immunol, Sch Med, Tokyo, Japan
关键词
allergic conjunctivitis; eosinophil; B7-H3; Th1; Th2;
D O I
10.1016/j.imlet.2007.07.011
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
B7-H3 negatively regulates Th1-mediated immune responses. Here, we aimed to investigate whether B7-H3 is involved in the development of murime experimental allergic conjunctivitis (EC), which is predominantly mediated by Th2 cells. Intraperitoneal injection of anti-B7-H3 Ab during the induction phase of EC significantly augmented the severity of EC evaluated as conjunctival eosinophil numbers and Ag-induced IL-5 production by splenocytes. Injection of anti-B7-H3 Ab during the effector phase of EC did not significantly affect the severity of EC. In addition, transfer of Ag-primed splenocytes treated with anti-B7-H3 Ab in vitro did not significantly affect the severity of EC, compared to the splenocytes treated with the control Ab. Thus, regulation of EC by blocking of B7-H3 was observed during the induction phase but not the effector phase. Moreover. this study provides a new notion that B7-H3 regulates not only Th1-mediated but also Th2-mediated immune reactions. (c) 2007 Published by Elsevier B.V.
引用
收藏
页码:52 / 57
页数:6
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