Comprehensive two-dimensional liquid chromatography applying two parallel columns in the second dimension

被引：62

作者：

Francois, Isabelle ^{[2
]}

de Villiers, Andre ^{[3
]}

Tienpont, Bart ^{[1
]}

David, Frank ^{[1
,2
]}

Sandra, Pat ^{[1
,2
,3
]}

机构：

[1] Res Inst Chromatog, B-8500 Kortrijk, Belgium

[2] Univ Ghent, Dept Organ Chem, B-9000 Ghent, Belgium

[3] Univ Stellenbosch, Dept Chem, ZA-7602 Matieland, South Africa

来源：

JOURNAL OF CHROMATOGRAPHY A | 2008年 / 1178卷 / 1-2期

关键词：

comprehensive two-dimensional LC; normal phase LC; reversed phase LC; peak capacity; two parallel second dimension columns;

D O I：

10.1016/j.chroma.2007.11.032

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The design of a new interface for comprehensive two-dimensional liquid chromatography (LC x LC) is described. To the conventionally used LC x LC system with the loop-type interface consisting of a two-position/ten-port switching valve equipped with two loops, an extra two-position/ten-port switching valve, a detector, a pump and a second column placed in parallel with the column in the second dimension, are added. The features of the interface are that the separation space in the second dimension is significantly enlarged and that the number of fractions transferred from the first to the second dimension can be increased, reducing the risk to lose resolution of the primary dimension. The potential of the system in NPLC x 2RPLC is illustrated with the analysis of a standard mixture and a lemon oil extract. For the lemon oil analysis, the effective peak capacity was increased from 437 using a conventional interface to 1095 with the new interface. RPLC x 2RPLC in combination with reduced modulation times was applied to the analysis of steroids and to the detection of impurities at the 0.05% relative concentration level in a suffonamide drug sample. (c) 2007 Published by Elsevier B.V.

引用

页码：33 / 42

页数：10

共 62 条

[31] High-efficiency liquid chromatography on conventional columns and instrumentation by using temperature as a variable I.: Experiments with 25 cm x 4.6 mm ID, 5 μm ODS columns [J].