Cytogenetic localization of 44 new coding sequences in the horse

被引：22

作者：

Godard, S ^{[1
]}

Vaiman, A ^{[1
]}

Schibler, L ^{[1
]}

Mariat, D ^{[1
]}

Vaiman, D ^{[1
]}

Cribiu, EP ^{[1
]}

Guérin, G ^{[1
]}

机构：

[1] INRA, Dept Denet Anim, Lab Genet Biochim & Cytogenet, Ctr Rech Jouy, F-78352 Jouy En Josas, France

来源：

MAMMALIAN GENOME | 2000年 / 11卷 / 12期

关键词：

D O I：

10.1007/s003350010206

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The purpose of this study was to increase the number of genes assigned by in situ hybridization to equine chromosomes and thus the number of links for comparative mapping with other species. Forty-four new sequences were added to the horse cytogenetic map by FISH mapping of BAC clones containing genes (35) or ESTs (9). Three approaches were developed: use of horst BAC clones screened with (i) horse EST primers, (ii) interspecific consensus intraexonic primers, and (iii) use of goat BAC containing genes previously localized on goat chromosomes. Present data suggest that the second approach is the most promising. A total of 46 segments containing one or several genes could be compared, among which 40 loci could be included in 16 synteny groups between human and horse, displaying one ordered segment and several breaking points along chromosomes. All single BAC localizations confirm the most recent mapping data. Twenty-six out of 31 chromosomes now contain a gene mapped by in situ hybridization, and 14 new arm-to-arm segment homologies were revealed.

引用

页码：1093 / 1097

页数：5

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